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Systematic name YPL068C
Gene name
Feature type ORF, Uncharacterized
Coordinates Chr XVI:425096..424215
Primary SGDID S000005989

Description of YPL068C: Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS[1][2]

Community Commentary

About Community Commentary. Please share your knowledge!

Ultraviolet Sensitivity

YPL068C UV light.jpeg BY4735(wild) uv light.jpg

YPL068C is more sensitive than BY4735(wild) given that there is less colonizes present when under the same stress.

This gene is part of the UW-Stout Orphan Gene Project. Learn more here.

Growth Curve

ypl068 growth.png

In a BY4735 background, knocking out YPL068C seems to have a moderate effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 124 minutes, while the YPL068C knock-out strain's doubling time was 209 minutes.

G-418 Stress


In the BY4735 background, knocking out YPL068C seems to have a little effect on the growth rate. In the knock-out experiment, the BY4735 strain's doubling time was 149 minutes, whereas the YPL068C knock-out strain's doubling time was 235 minutes. The calibration experiment, the BY4735 strain's doubling time was 64 minutes, whereas the YPL068C knock-out strain's doubling time was 92 minutes.

Heat Shock


Heat shock had no change on the doubling time, but the starting amount of yeast cells less. The heat seemed to kill off the cells, the longer they were in the hot water bath.

Methanol Sensitivity

The wild type had a doubling time of 150 min, the YPL068c had a doubling time of 200 min. The doubling time increased which means this strain is sensitive to methanol.


YPL068C Methanol sensitivity.png

Stressing with Hydroxyurea

Hydroxyurea Protocol -The wild type strain had only a small difference in doubling time when stressed with Hydroxyurea;YPL068C showed about a 30 minute difference in doubling time compared to the wild type strain, indicating that this gene most likely plays some role in DNA replication.

HU YPL068C.png


Fermentation protocol link [2] - There appears to be no major difference in fermentation rate. The lower final ethanol percentage could be a result of evaporation. If this experiment were to be carried out again then it should be done in an air tight container. Fer YPL068C.PNG

Caffeine Sensitivity

YPL068C VS BY4735 Caffeine PRotocol [[UW-Stout/Caffeine], the genes reacted the same at the beginning, varied in the middle then came back together at the end.

Stressing with pH

pH stress protocol link [3]

When I looked at the data this graph shows I see that both the Wild type and also the yeast strain YPL068C have about the same linear pathway and so I came to the conclusion that even though the yeast strain YPL068C has somewhat a deviation difference but its still small enough and somewhat close enough to the wild types and so therefore both of them are yet resilient to the pH 4 stress test.


Cold Shock Sensitivity

File:YPL068C.png Cold Shock Protocol [[4]]


See Help:References on how to add references

  1. Huh WK, et al. (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91 SGD PMID 14562095
  2. Lee MW, et al. (2007) Global protein expression profiling of budding yeast in response to DNA damage. Yeast 24(3):145-54 SGD PMID 17351896

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