Difference between revisions of "YPL068C"

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(Community Commentary)
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===[[UW-Stout/Sensitivity To Nitrogen Starvation]]===
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[[File: YPL065C_growth plate1.JPG|thumb|Growth of YPL065C on synthetic defined media (SDC).]]
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[[File: YPL065C_starvation plate.JPG|thumb|Growth of YFL064C on omission media that lacks nitrogen.]]
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[[File: YPL065C_revival plate.JPG|thumb|Growth of transferred BY4735 on Synthetic defined media.]]
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Knocking out the YPL068C seems to have no effect on growth after incubating for 5 days on the Nitrogen omitted media.
  
 
===[[UW-Stout/Ultraviolet Light|Ultraviolet Sensitivity]]===
 
===[[UW-Stout/Ultraviolet Light|Ultraviolet Sensitivity]]===

Revision as of 07:38, 2 May 2019

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Systematic name YPL068C
Gene name
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr XVI:425096..424215
Primary SGDID S000005989


Description of YPL068C: Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus and is induced in response to the DNA-damaging agent MMS[1][2]




Community Commentary

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UW-Stout/Sensitivity To Nitrogen Starvation

File:YPL065C growth plate1.JPG
Growth of YPL065C on synthetic defined media (SDC).
Growth of YFL064C on omission media that lacks nitrogen.
Growth of transferred BY4735 on Synthetic defined media.

Knocking out the YPL068C seems to have no effect on growth after incubating for 5 days on the Nitrogen omitted media.

Ultraviolet Sensitivity

YPL068C UV light.jpeg BY4735(wild) uv light.jpg

YPL068C is more sensitive than BY4735(wild) given that there is less colonizes present when under the same stress.


This gene is part of the UW-Stout Orphan Gene Project. Learn more here.

Growth Curve

ypl068 growth.png

In a BY4735 background, knocking out YPL068C seems to have a moderate effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 124 minutes, while the YPL068C knock-out strain's doubling time was 209 minutes.


image008.png

Heat Shock

HeatShockforYP.png



References

See Help:References on how to add references

  1. Huh WK, et al. (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91 SGD PMID 14562095
  2. Lee MW, et al. (2007) Global protein expression profiling of budding yeast in response to DNA damage. Yeast 24(3):145-54 SGD PMID 17351896

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