Difference between revisions of "YOR027W"

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=== Interactions ===
 
[[Category:Topic:Interactions]]
 
==== Regulatory ====
 
[[Category:Topic:Interactions:Regulatory]]
 
Regulatory interaction with Ssa1 [[Category:Regulatory Interactions with Ssa1]]<br>
 
Binding of Sti1 to Ssa1 activates the ATPase of Ssa1 by a factor of about 200, which is in contrast to the behaviour of Hop in the mammalian Hsp70 system. Analysis of the underlying activation mechanism revealed that ATP hydrolysis is rate limiting in the Ssa1 ATPase cycle and that this step is accelerated by Sti1. <ref name='S000073412'>Wegele H, et al. (2003) Sti1 is a novel activator of the Ssa proteins. J Biol Chem 278(28):25970-6 {{SGDpaper|S000073412}} PMID 12716905</ref> <ref name = 'CAset9598-2003-09-03'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=9598 Dr. Harald Wegele] on 2003-09-03</ref>
 
 
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==== Two Hybrid ====
 
[[Category:Topic:Interactions:Two Hybrid]]
 
Two Hybrid interaction with Ssa1 [[Category:Two Hybrid Interactions with Ssa1]]<br>
 
With a two hybrid approach and coimmunoprecipitations, we show that Sti1 specifically interacts with the Ssa group of the cytosolic yeast Hsp70 proteins and not with the Ssb, Sse and Ssz group. <ref name='S000073412'>Wegele H, et al. (2003) Sti1 is a novel activator of the Ssa proteins. J Biol Chem 278(28):25970-6 {{SGDpaper|S000073412}} PMID 12716905</ref> <ref name = 'CAset9598-2003-09-03'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=9598 Dr. Harald Wegele] on 2003-09-03</ref>
 
 
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==Community Commentary==
 
==Community Commentary==

Revision as of 11:51, 24 January 2007

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Systematic name YOR027W
Gene name STI1
Aliases
Feature type ORF, Verified
Coordinates Chr XV:381053..382822
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Description of YOR027W: Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones; activates the ATPase activity of Ssa1p; homolog of mammalian Hop protein[1][2][3][4]



Community Commentary

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Interactions

Regulatory

Regulatory interaction with Ssa1
Binding of Sti1 to Ssa1 activates the ATPase of Ssa1 by a factor of about 200, which is in contrast to the behaviour of Hop in the mammalian Hsp70 system. Analysis of the underlying activation mechanism revealed that ATP hydrolysis is rate limiting in the Ssa1 ATPase cycle and that this step is accelerated by Sti1. [1] [5]


Two Hybrid

Two Hybrid interaction with Ssa1
With a two hybrid approach and coimmunoprecipitations, we show that Sti1 specifically interacts with the Ssa group of the cytosolic yeast Hsp70 proteins and not with the Ssb, Sse and Ssz group. [1] [5]


References

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  1. 1.0 1.1 1.2 Wegele H, et al. (2003) Sti1 is a novel activator of the Ssa proteins. J Biol Chem 278(28):25970-6 SGD PMID 12716905
  2. Richter K, et al. (2003) Sti1 is a non-competitive inhibitor of the Hsp90 ATPase. Binding prevents the N-terminal dimerization reaction during the atpase cycle. J Biol Chem 278(12):10328-33 SGD PMID 12525481
  3. Chang HC, et al. (1997) In vivo analysis of the Hsp90 cochaperone Sti1 (p60). Mol Cell Biol 17(1):318-25 SGD PMID 8972212
  4. Nicolet CM and Craig EA (1989) Isolation and characterization of STI1, a stress-inducible gene from Saccharomyces cerevisiae. Mol Cell Biol 9(9):3638-46 SGD PMID 2674681
  5. 5.0 5.1 submitted by Dr. Harald Wegele on 2003-09-03

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