Difference between revisions of "YKR005C"

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The protocol can be found at  
 
The protocol can be found at  
 
===[[UW-Stout/Caffeine_FA19|Caffeine]]===
 
===[[UW-Stout/Caffeine_FA19|Caffeine]]===
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===[[UW-Stout/Prednisone FA19|Prednisone Effects on Yeast Growth]]===
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[[File:YKR005C.png|750 px]]
  
 
<!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation -->
 
<!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation -->

Revision as of 08:47, 12 December 2019

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Systematic name YKR005C
Gene name
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr XI:450167..448521
Primary SGDID S000001713


Description of YKR005C: Putative protein of unknown function[1]




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Community Commentary

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This gene is part of the UW-Stout Orphan Gene Project. Learn more here.

Growth in YPD

YKR005C YPD.png

In a BY4735 background, knocking out YKR005C seems to have a little to no effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 414 minutes, while the YKR005C knock-out strain's doubling time was 427 minutes. (These doubling times are the means of three experiments.)

Caffeine and Yeast Cells

megan29.png

This graph shows the growth curve with and without caffeine. It has the knockout strain and wild type yeast cells. The caffeine enhanced the growth in both wild type and knockout strain. The average doubling time between the three experiments for the knockout strand was 736.38 minutes. The caffeine also had a positive effect on the growth curve for the wild type with the average doubling time being 1245.02 minutes. The results were concluded from the wild type and knockout strand was with this amount of caffeine it leads to an increase in growth.

The protocol can be found at

Caffeine

Prednisone Effects on Yeast Growth

YKR005C.png



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References

See Help:References on how to add references

  1. Dusterhoft A and Philippsen P (1992) DNA sequencing and analysis of a 24.7 kb segment encompassing centromere CEN11 of Saccharomyces cerevisiae reveals nine previously unknown open reading frames. Yeast 8(9):749-59 SGD PMID 1441752

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