YJL133C-A

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Systematic name	YJL133C-A
Gene name
Aliases
Feature type	ORF, Uncharacterized
Coordinates	Chr X:159847..159623
Primary SGDID	S000028805

Description of YJL133C-A: Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies




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Community Commentary

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Caffeine

Interpretation

The average doubling time for the YJL133C-A (C2) gene in standard conditions was 89.5 minutes. Under caffeine stress, the average doubling time was 134 minutes. That is a 49.7% increase in doubling time which means that cell growth was negatively affected (slowed down) by this type of stress.

Ethanol

YJL133C-A KO gene

UV Light

Wild type yeast prepared according to sample prep. No UV exposure. 72 colonies.

Wild type yeast prepared according to sample prep and UV exposure protocol. 11 colonies.

YJL133C-A knock-out yeast prepared according to sample prep. No UV exposure. 68 colonies.

YJL133C-A knock-out yeast prepared according to sample prep and UV exposure protocol. Trial 1. 13 colonies.

YJL133C-A knockout yeast prepared according to sample prep and UV exposure protocol. Trial 2. 7 colonies.

The plate with the modified yeast that was not exposed to UV had approximately 5% fewer colonies than the wild-type plate that was not exposed to UV. This shows that the knock-out strain of yeast is expected to be less viable compared to wild-type yeast in the absence of stressors. The two trial plates were pretty similar in colony count. The average of the two was 10 colonies. The plate with wild-type yeast that was exposed to UV had 11 colonies. There was no significant difference in colony count between the modified yeast and wild-type yeast that was exposed to UV.

As there was no significant difference between the wild-type yeast exposed to UV and the trial strains, we can conclude that the gene is not used in the defense against UV damage nor the repair of UV damage in yeast.

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UW Stout/FA 2022 Glycerol Media

Introduction

Following the UW-Stout/Glycerol FA22 protocol, 5 knockout strains of yeast cell were tortured within a glycerol media solution.

Results

The following doubling times were calculated:

• Negative Control Trial 1: 163 minutes
• Glycerol Trial 2: 565 minutes
• Negative Control Trial 2:
• Glycerol Trial 3:
• Negative Control Trial 3: