YJL043W

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Systematic name	YJL043W
Gene name
Aliases
Feature type	ORF, Uncharacterized
Coordinates	Chr X:360130..360903
Primary SGDID	S000003579

Description of YJL043W: Putative protein of unknown function; YJL043W is a non-essential gene

Community Commentary

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UW-Stout/Sensitivity To Nitrogen Starvation

Growth of YJLO43W on synthetic defined media (SDC).

Growth of YJLO43W on omission media that lacks nitrogen.

Growth of transferred BY4735 on Synthetic defined media.

Knocking out the YJL043W seems to have no effect on growth after incubating for 5 days on the Nitrogen omitted media.

Ultraviolet Sensitivity

YJL043W is more sensitive than BY4735(wild) given that there is less colonizes present when under the same stress.

This gene is part of the UW-Stout Orphan Gene Project. Learn more here.

Growth Curve

In a BY4735 background, knocking out YJL043W seems to have little to no effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 124 minutes, while the YJL043W knock-out strain's doubling time was 155 minutes.

G-418 Stress

In a BY4735 background, knocking out YJL043W seems to have a small effect on the growth, the difference was less than BY4735. In the knock-out experiment, the BY4735 strain's doubling time was 149 minutes, while the YJL043W knock-out strain's doubling time was 237 minutes. In the calibration experiment, the BY4735 strain's doubling time was 64 minutes, while the YJL043W knock out strain's doubling time was 185 minutes.

Methanol Sensitivity

Heat Shock

Heat shock had no change on the doubling time, but the starting amount of yeast cells less. The heat seemed to kill off the cells, the longer they were in the hot water bath.

Stressing with Hydroxyurea

Hydroxyurea Protocol -The wild type strain had only a small difference in doubling time when stressed with Hydroxyurea;YJL043W showed about a 20 minute difference of doubling time from the wild type strain when stressed with hydroxyurea, indicating that this gene most likely does not play a vital role in DNA replication.

Fermentation

Fermentation protocol link [1] - There appears to be no major difference in fermentation rate. The lower final ethanol percentage could be a result of evaporation. If this experiment were to be carried out again then it should be done in an air tight container.

Caffeine Sensitivity

Caffeine Protocol UW-Stout/Caffeine, results very similar, hardly any difference in pace.

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Stressing with pH

pH stress protocol link [2]

The yeast strain YJL043W had somewhat of a significant difference compared to the wild type even though the wild type keeps growing but not as fast as the yeast strain YJL043W does and the YJL043W strain does plato's at somewhat the middle and then continues to plummets. But wild type keeps slowly climbing at a steady rate of growth and time. So, Therefore we have made a decision that the wild type strain is more resilient to the pH level of 4 we tested it at then the YJL043W Strain is.

Cold Shock Sensitivity

Cold Shock Protocol [[3]]

Protocol: Cold Shock

The Results from this graph have proven that compared to the wild type there was no significant change in the doubling time. This has proven that its neither more or less sensitive in comparison to the wild type.

References

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