Difference between revisions of "YJL043W"

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(G-418 Stress)
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===[[UW-Stout/G-418 | G-418 Stress]]===
 
===[[UW-Stout/G-418 | G-418 Stress]]===
  
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In a BY4735 background, knocking out YJL043W seems to have a small effect on the growth, the difference was less than BY4735. In the knock-out experiment, the BY4735 strain's doubling time was 149 minutes, while the YJL043W knock-out strain's doubling time was 237 minutes. In the calibration experiment, the BY4735 strain's doubling time was 64 minutes, while the YJL043W knock out strain's doubling time was 185 minutes.  
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In a BY4735 background, knocking out YJL043W seems to have a small effect on the growth, the difference was less than BY4735. In the knock-out experiment, the BY4735 strain's doubling time was 149 minutes, while the YJL043W knock-out strain's doubling time was 237 minutes. In the calibration experiment, the BY4735 strain's doubling time was 64 minutes, while the YJL043W knock out strain's doubling time was 185 minutes.
  
 
===Methanol Sensitivity===
 
===Methanol Sensitivity===

Revision as of 18:13, 5 May 2019

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Systematic name YJL043W
Gene name
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr X:360130..360903
Primary SGDID S000003579


Description of YJL043W: Putative protein of unknown function; YJL043W is a non-essential gene[1][2]




Community Commentary

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UW-Stout/Sensitivity To Nitrogen Starvation

Growth of YJLO43W on synthetic defined media (SDC).
Growth of YJLO43W on omission media that lacks nitrogen.
Growth of transferred BY4735 on Synthetic defined media.

Knocking out the YJL043W seems to have no effect on growth after incubating for 5 days on the Nitrogen omitted media.


Ultraviolet Sensitivity

YJL043W uv light.jpg BY4735(wild) uv light.jpg

YJL043W is more sensitive than BY4735(wild) given that there is less colonizes present when under the same stress.


This gene is part of the UW-Stout Orphan Gene Project. Learn more here.

Growth Curve

yjl043w growth.png

In a BY4735 background, knocking out YJL043W seems to have little to no effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 124 minutes, while the YJL043W knock-out strain's doubling time was 155 minutes.

G-418 Stress

image018.png

In a BY4735 background, knocking out YJL043W seems to have a small effect on the growth, the difference was less than BY4735. In the knock-out experiment, the BY4735 strain's doubling time was 149 minutes, while the YJL043W knock-out strain's doubling time was 237 minutes. In the calibration experiment, the BY4735 strain's doubling time was 64 minutes, while the YJL043W knock out strain's doubling time was 185 minutes.

Methanol Sensitivity

YJL043W Methanol sensitivity.png

Stressing with Hydroxyurea

Hydroxyurea Protocol -The wild type strain had only a small difference in doubling time when stressed with Hydroxyurea;YJL043W showed about a 20 minute difference of doubling time from the wild type strain when stressed with hydroxyurea, indicating that this gene most likely does not play a vital role in DNA replication.

HU YJLO43W.png

Fermentation

Fermentation protocol link [1] - There appears to be no major difference in fermentation rate. The lower final ethanol percentage could be a result of evaporation. If this experiment were to be carried out again then it should be done in an air tight container.

Fer YJL043W.PNG


References

See Help:References on how to add references

  1. Entian KD, et al. (1999) Functional analysis of 150 deletion mutants in Saccharomyces cerevisiae by a systematic approach. Mol Gen Genet 262(4-5):683-702 SGD PMID 10628851
  2. Giaever G, et al. (2002) Functional profiling of the Saccharomyces cerevisiae genome. Nature 418(6896):387-91 SGD PMID 12140549

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