Difference between revisions of "YHR094C"

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|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Systematic name''' || [http://db.yeastgenome.org/cgi-bin/locus.pl?dbid=S000001136 YHR094C]  
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|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Systematic name''' || [http://www.yeastgenome.org/cgi-bin/locus.pl?dbid=S000001136 YHR094C]  
 
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|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Gene name'''        ||''HXT1 ''
 
|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Gene name'''        ||''HXT1 ''
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|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Coordinates'''
 
|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Coordinates'''
|nowrap| Chr VIII:292628..290916
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|nowrap| Chr VIII:292625..290913
 
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|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Primary SGDID'''          || S000001136
 
|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Primary SGDID'''          || S000001136

Latest revision as of 07:45, 23 January 2012

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Systematic name YHR094C
Gene name HXT1
Aliases HOR4
Feature type ORF, Verified
Coordinates Chr VIII:292625..290913
Primary SGDID S000001136


Description of YHR094C: Low-affinity glucose transporter of the major facilitator superfamily, expression is induced by Hxk2p in the presence of glucose and repressed by Rgt1p when glucose is limiting[1][2]




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Community Commentary

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Alleles, Strains, and Phenotypes

Multiple Knockout Strains

Strain Background: CEN.PK2-1C
Genotype: EBY4000 (hxt)
Together with: HXT15, MPH2, HXT7, HXT6, HXT3, HXT13, HXT10, MAL11, HXT4, HXT5, HXT12, HXT8, HXT9, HXT16, MPH3, GAL2, HXT2, HXT14, HXT17, HXT11
Phenotype(s): Viable , Conditionally inviable when on glucose medium

The complete hxt null strain (EBY4000) is unable to take up hexoses and to grow on them. It is well suited to express and characterize heterologous glucose transporters. [3] [4]


DNA and RNA Details

Other DNA and RNA Details

Other Topic: expression

Specifically lower expression in carbon limited chemostat cultures versus carbon excess. [5] [6]





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References

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  1. Mosley AL, et al. (2003) Glucose-mediated phosphorylation converts the transcription factor Rgt1 from a repressor to an activator. J Biol Chem 278(12):10322-7 SGD PMID 12527758
  2. Rodriguez A, et al. (2001) The hexokinase 2 protein regulates the expression of the GLK1, HXK1 and HXK2 genes of Saccharomyces cerevisiae. Biochem J 355(Pt 3):625-31 SGD PMID 11311123
  3. Wieczorke R, et al. (1999) Concurrent knock-out of at least 20 transporter genes is required to block uptake of hexoses in Saccharomyces cerevisiae. FEBS Lett 464(3):123-8 SGD PMID 10618490
  4. submitted by Eckhard Boles on 2003-03-17
  5. Boer VM, et al. (2003) The genome-wide transcriptional responses of Saccharomyces cerevisiae grown on glucose in aerobic chemostat cultures limited for carbon, nitrogen, phosphorus, or sulfur. J Biol Chem 278(5):3265-74 SGD PMID 12414795
  6. submitted by Viktor Boer on 2003-07-25

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