YHR048W

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Systematic name YHR048W
Gene name YHK8
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr VIII:204607..206151
Primary SGDID S000001090


Description of YHR048W: Presumed antiporter of the DHA1 family of multidrug resistance transporters; contains 12 predicted transmembrane spans; expression of gene is up-regulated in cells exhibiting reduced susceptibility to azoles[1][2]




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Community Commentary

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DNA and RNA Details

Other DNA and RNA Details

Other Topic: expression

Specifically lower expression in carbon limited chemostat cultures versus carbon excess. [3] [4]


Growth in YPD

YHR048W YPD.png

In a BY4735 background, knocking out YHR048 seems to have little to no effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 414 minutes, while the YHR048W knock-out strain's doubling time was 432 minutes. (These doubling times are the means of three experiments.)

Caffeine and Yeast Cells

megan26.png

This graph shows the growth curve with and without caffeine. It has the knockout strain and wild type yeast cells. The caffeine enhanced the growth in both wild type and knockout strain. The average doubling time between the three experiments for the knockout strand was 801.97 minutes. The caffeine also had a positive effect on the growth curve for the wild type with the average doubling time being 1245.02 minutes. The results were concluded from the wild type and knockout strand was with this amount of caffeine it leads to an increase in growth.

The protocol can be found at

Caffeine

yHR048cUV Exposure.png

This bar graph shows that compared to a BY4735 background, Knocking out yHR048c makes yeast less sensitive to UV light exposure

The protocol can be found at

UW_Stout/UV_Light_FA19UV Light Exposure

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References

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  1. Barker KS, et al. (2003) Identification of genes differentially expressed in association with reduced azole susceptibility in Saccharomyces cerevisiae. J Antimicrob Chemother 51(5):1131-40 SGD PMID 12697649
  2. Gbelska Y, et al. (2006) Evolution of gene families: the multidrug resistance transporter genes in five related yeast species. FEMS Yeast Res 6(3):345-55 SGD PMID 16630275
  3. Boer VM, et al. (2003) The genome-wide transcriptional responses of Saccharomyces cerevisiae grown on glucose in aerobic chemostat cultures limited for carbon, nitrogen, phosphorus, or sulfur. J Biol Chem 278(5):3265-74 SGD PMID 12414795
  4. submitted by Viktor Boer on 2003-07-25

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