Difference between revisions of "YHR048W"

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(Effect of 3% DMSO on YHR048W)
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===Effect of 3% DMSO on YHR048W===
===Effect of 3% [[UW Stout/DMSO FA19|DMSO]] on YHR048W===

Revision as of 17:24, 13 December 2019

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Systematic name YHR048W
Gene name YHK8
Feature type ORF, Uncharacterized
Coordinates Chr VIII:204607..206151
Primary SGDID S000001090

Description of YHR048W: Presumed antiporter of the DHA1 family of multidrug resistance transporters; contains 12 predicted transmembrane spans; expression of gene is up-regulated in cells exhibiting reduced susceptibility to azoles[1][2]


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DNA and RNA Details

Other DNA and RNA Details

Other Topic: expression

Specifically lower expression in carbon limited chemostat cultures versus carbon excess. [3] [4]

Growth in YPD

YHR048W YPD.png

In a BY4735 background, knocking out YHR048 seems to have little to no effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 414 minutes, while the YHR048W knock-out strain's doubling time was 432 minutes. (These doubling times are the means of three experiments.)

Caffeine and Yeast Cells


This graph shows the growth curve with and without caffeine. It has the knockout strain and wild type yeast cells. The caffeine enhanced the growth in both wild type and knockout strain. The average doubling time between the three experiments for the knockout strand was 801.97 minutes. The caffeine also had a positive effect on the growth curve for the wild type with the average doubling time being 1245.02 minutes. The results were concluded from the wild type and knockout strand was with this amount of caffeine it leads to an increase in growth.

The protocol can be found at


yHR048cUV Exposure.png

This bar graph shows that compared to a BY4735 background, Knocking out yHR048c makes yeast less sensitive to UV light exposure

The protocol can be found at

UW_Stout/UV_Light_FA19UV Light Exposure

Cycloheximide effect on YHR048W

Cycloheximide 8.png

As you can see from above test 4 thrived, it did very good, but test 1-3 did not do as well and were all lower than the growth curve. Growth models 1-3 are YHR048W without any stress induced.

Effect of 3% DMSO on YHR048W

Effect of 3% DMSO on YHR048W.png

The graph above shows the effect of 3% DMSO on YHR048W. From the data represented the average doubling rate for the YHR048W knockout strain without DMSO was 575 minutes. When DMSO is added the average doubling time increased to 1851 minutes. This shows that 3% DMSO did add stress to this knockout strain. The average doubling time of WT:BY4735 without DMSO was 514 minutes. The average doubling rate for WT:BY4735 with DMSO was 1529 minutes. If we compare the average doubling rate of WT:BY4735 with DMSO to YHR048W with DMSO we can see that without the YHR048W gene the yeast was unable to grow as quickly. This suggest that the YHR048W gene does play a role in protecting yeast against oxidative stress induced by DMSO. If we compare the growth rates of the controls for both WT:BY4735 and YHR048W we can see that the YHR048W gene barely effects the growth of yeast in optimal conditions.

Ethanol Tolerance in YHR048W

YHR048W ethonal.png

This graph shows the growth between YHR048W treated and untreated with ethanol. The rate of growth of the yeast treated with ethanol is (answer). The rate of growth of the yeast not treated with ethanol is (answer). This shows that the strands of YHR048W treated with ethanol have an increased growth rate than the ones that weren't.

Prednisone Effects on YHR048W




See Help:References on how to add references

  1. Barker KS, et al. (2003) Identification of genes differentially expressed in association with reduced azole susceptibility in Saccharomyces cerevisiae. J Antimicrob Chemother 51(5):1131-40 SGD PMID 12697649
  2. Gbelska Y, et al. (2006) Evolution of gene families: the multidrug resistance transporter genes in five related yeast species. FEMS Yeast Res 6(3):345-55 SGD PMID 16630275
  3. Boer VM, et al. (2003) The genome-wide transcriptional responses of Saccharomyces cerevisiae grown on glucose in aerobic chemostat cultures limited for carbon, nitrogen, phosphorus, or sulfur. J Biol Chem 278(5):3265-74 SGD PMID 12414795
  4. submitted by Viktor Boer on 2003-07-25

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