Difference between revisions of "YHR048W"

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Caffeine and Strand YHR048W
 
 
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Caffeine and Wild Type
 
 
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In a BY4735 background, knocking out YHR048 seems to have little to no effect on growth rate in log-phase.  In this assay, the BY4735 strain's doubling time was 414 minutes, while the YHR048W knock-out strain's doubling time was 432 minutes.  (These doubling times are the means of three experiments.)
 
In a BY4735 background, knocking out YHR048 seems to have little to no effect on growth rate in log-phase.  In this assay, the BY4735 strain's doubling time was 414 minutes, while the YHR048W knock-out strain's doubling time was 432 minutes.  (These doubling times are the means of three experiments.)
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Caffeine and Yeast Cells
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Revision as of 07:43, 12 December 2019

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Systematic name YHR048W
Gene name YHK8
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr VIII:204607..206151
Primary SGDID S000001090


Description of YHR048W: Presumed antiporter of the DHA1 family of multidrug resistance transporters; contains 12 predicted transmembrane spans; expression of gene is up-regulated in cells exhibiting reduced susceptibility to azoles[1][2]




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Community Commentary

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DNA and RNA Details

Other DNA and RNA Details

Other Topic: expression

Specifically lower expression in carbon limited chemostat cultures versus carbon excess. [3] [4]


Growth in YPD

YHR048W YPD.png

In a BY4735 background, knocking out YHR048 seems to have little to no effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 414 minutes, while the YHR048W knock-out strain's doubling time was 432 minutes. (These doubling times are the means of three experiments.)

Caffeine and Yeast Cells

megan26.png



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References

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  1. Barker KS, et al. (2003) Identification of genes differentially expressed in association with reduced azole susceptibility in Saccharomyces cerevisiae. J Antimicrob Chemother 51(5):1131-40 SGD PMID 12697649
  2. Gbelska Y, et al. (2006) Evolution of gene families: the multidrug resistance transporter genes in five related yeast species. FEMS Yeast Res 6(3):345-55 SGD PMID 16630275
  3. Boer VM, et al. (2003) The genome-wide transcriptional responses of Saccharomyces cerevisiae grown on glucose in aerobic chemostat cultures limited for carbon, nitrogen, phosphorus, or sulfur. J Biol Chem 278(5):3265-74 SGD PMID 12414795
  4. submitted by Viktor Boer on 2003-07-25

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