Difference between revisions of "YHR033W"

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(Ethanol)
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===[[UW-Stout/Ethanol_FA22|Ethanol]]===
 
===[[UW-Stout/Ethanol_FA22|Ethanol]]===
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[[Image:Best Ethanol KO graph.png|Graph|center|700px|YJL133C-A]]
  
 
===[[UW-Stout/Caffeine_FA22|Caffeine]]===
 
===[[UW-Stout/Caffeine_FA22|Caffeine]]===

Revision as of 08:26, 17 December 2022

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Systematic name YHR033W
Gene name
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr VIII:175548..176819
Primary SGDID S000001075


Description of YHR033W: Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm[1]




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Community Commentary

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Ethanol

YJL133C-A

Caffeine

Control

Trial 1

Trial 2

Interpretation

The average doubling time for the YHR033W (C5) gene in standard conditions was 87 minutes. Under caffeine stress, the average doubling time was 165.5 minutes. That is a 90.2% increase in doubling time which means that cell growth was negatively affected (slowed down) by this type of stress.



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References

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  1. Kumar A, et al. (2002) Subcellular localization of the yeast proteome. Genes Dev 16(6):707-19 SGD PMID 11914276

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UW Stout/FA 2022 Glycerol Media

Introduction

Following the UW-Stout/Glycerol FA22 protocol, 5 knockout strains of yeast cell were tortured within a glycerol media solution.

Results

Knockout 4 Trial 1.png Negative Control KO4 Trial 1.png Knockout 4 Trial 2.png Negative Control KO4 Trial 2.png Growth Curve KO4 Trial 3.png Negative Control KO4 Trial 3.png

The following doubling times were calculated:

  • Glycerol Trial 1: NA
  • Negative Control Trial 1: 199 minutes
  • Glycerol Trial 2: 526 minutes
  • Negative Control Trial 2: 215 minutes
  • Glycerol Trial 3:2531 minutes
  • Negative Control Trial 3: 277 minutes

Analysis/Conclusion