Difference between revisions of "YHR033W"
(→Caffeine) |
|||
| (24 intermediate revisions by the same user not shown) | |||
| Line 31: | Line 31: | ||
===[[UW-Stout/Ethanol_FA22|Ethanol]]=== | ===[[UW-Stout/Ethanol_FA22|Ethanol]]=== | ||
| + | |||
| + | [[Image:Best Ethanol KO graph.png|Graph|center|700px|YJL133C-A]] | ||
| + | YHR033W is slightly above YJL133C-A in terms of growth but has a similar curve. The doubling time is 76.63. Ethanol didn't affect the growth. | ||
===[[UW-Stout/Caffeine_FA22|Caffeine]]=== | ===[[UW-Stout/Caffeine_FA22|Caffeine]]=== | ||
| Line 38: | Line 41: | ||
[[Image:Knockout_v._caffeine_trial1_Viv.png|100pxs|Trial 1]] | [[Image:Knockout_v._caffeine_trial1_Viv.png|100pxs|Trial 1]] | ||
| − | [[Image:Knockout_gene_v._Caffeine_trial_2.png| | + | [[Image:Knockout_gene_v._Caffeine_trial_2.png|600px|Trial 2]] |
====Interpretation==== | ====Interpretation==== | ||
The average doubling time for the YHR033W (C5) gene in standard conditions was 87 minutes. Under caffeine stress, the average doubling time was 165.5 minutes. That is a 90.2% increase in doubling time which means that cell growth was negatively affected (slowed down) by this type of stress. | The average doubling time for the YHR033W (C5) gene in standard conditions was 87 minutes. Under caffeine stress, the average doubling time was 165.5 minutes. That is a 90.2% increase in doubling time which means that cell growth was negatively affected (slowed down) by this type of stress. | ||
| + | |||
| + | ===Hydrogen Peroxide=== | ||
| + | |||
| + | [[Image:2.jpg|center|thumb|600px|The x-axis is time in minutes and the y-axis is OD600]] | ||
| + | |||
| + | This is the link to the protocol page: [[UW-Stout/Hydrogen Peroxide FA22]]. | ||
| + | |||
| + | The doubling time for the YHR033W gene when stressed is 375 minutes and without stress is 221 minutes. The BY4375 under stress had a 598 minute doubling time and with no stress there was a 229 minute doubling time. The YHR033W gene presented a doubling time that was much faster than the wild type yeast. This data demonstrates that the YHR033W gene was more resistant to the hydrogen peroxide than the wild type. There is also a chance this change to be due from experimental error such as not vortexing the wild type yeast or having other microorganisms on your gloves. When no stress was present, the wild type yeast and YHR003W had very similar doubling time. | ||
<!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation --> | <!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation --> | ||
| Line 59: | Line 70: | ||
{{RefHelp}} | {{RefHelp}} | ||
</protect> | </protect> | ||
| + | |||
| + | ==UW Stout/FA 2022 Glycerol Media== | ||
| + | ===Introduction=== | ||
| + | Following the [[UW-Stout/Glycerol FA22]] protocol, 5 knockout strains of yeast cell were tortured within a glycerol media solution. | ||
| + | ===Results=== | ||
| + | [[Image:Knockout_4_Trial_1.png|600px]] | ||
| + | [[Image:Negative_Control_KO4_Trial_1.png|600px]] | ||
| + | [[Image:Knockout_4_Trial_2.png|600px]] | ||
| + | [[Image:Negative_Control_KO4_Trial_2.png|600px]] | ||
| + | [[Image:Growth_Curve_KO4_Trial_3.png|600px]] | ||
| + | [[Image:Negative_Control_KO4_Trial_3.png|600px]] | ||
| + | |||
| + | The following doubling times were calculated: | ||
| + | * Glycerol Trial 1: NA | ||
| + | * Negative Control Trial 1: 199 minutes | ||
| + | * Glycerol Trial 2: 526 minutes | ||
| + | * Negative Control Trial 2: 215 minutes | ||
| + | * Glycerol Trial 3:2531 minutes | ||
| + | * Negative Control Trial 3: 277 minutes | ||
| + | |||
| + | ===Analysis/Conclusion=== | ||
| + | |||
| + | ==UW Stout/FA 2022 Bud Scars== | ||
| + | Cells that had the gene YHR033W had an average of 1.03 scars per cell. Out of 100 cells counted, 103 bud scars were counted. The wild yeast BY4735 had an average of 2.46 bud scars per cell (100 cells counted, 246 bud scars). This indicates that the knocked-out gene significantly slowed down the reproduction rate of these cells. | ||
| + | [[File:Yeast_4(2)_2_48.png]] | ||
| + | ''picture does not include all cells that were counted and added for a visual of bud scars'' | ||
Latest revision as of 17:45, 20 December 2022
Share your knowledge...Edit this entry! <protect>
| Systematic name | YHR033W |
| Gene name | |
| Aliases | |
| Feature type | ORF, Uncharacterized |
| Coordinates | Chr VIII:175548..176819 |
| Primary SGDID | S000001075 |
Description of YHR033W: Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm[1]
</protect>
Contents
Community Commentary
About Community Commentary. Please share your knowledge!
Ethanol
YHR033W is slightly above YJL133C-A in terms of growth but has a similar curve. The doubling time is 76.63. Ethanol didn't affect the growth.
Caffeine
Interpretation
The average doubling time for the YHR033W (C5) gene in standard conditions was 87 minutes. Under caffeine stress, the average doubling time was 165.5 minutes. That is a 90.2% increase in doubling time which means that cell growth was negatively affected (slowed down) by this type of stress.
Hydrogen Peroxide
This is the link to the protocol page: UW-Stout/Hydrogen Peroxide FA22.
The doubling time for the YHR033W gene when stressed is 375 minutes and without stress is 221 minutes. The BY4375 under stress had a 598 minute doubling time and with no stress there was a 229 minute doubling time. The YHR033W gene presented a doubling time that was much faster than the wild type yeast. This data demonstrates that the YHR033W gene was more resistant to the hydrogen peroxide than the wild type. There is also a chance this change to be due from experimental error such as not vortexing the wild type yeast or having other microorganisms on your gloves. When no stress was present, the wild type yeast and YHR003W had very similar doubling time.
<protect>
References
See Help:References on how to add references
See Help:Categories on how to add the wiki page for this gene to a Category </protect>
UW Stout/FA 2022 Glycerol Media
Introduction
Following the UW-Stout/Glycerol FA22 protocol, 5 knockout strains of yeast cell were tortured within a glycerol media solution.
Results
The following doubling times were calculated:
- Glycerol Trial 1: NA
- Negative Control Trial 1: 199 minutes
- Glycerol Trial 2: 526 minutes
- Negative Control Trial 2: 215 minutes
- Glycerol Trial 3:2531 minutes
- Negative Control Trial 3: 277 minutes
Analysis/Conclusion
UW Stout/FA 2022 Bud Scars
Cells that had the gene YHR033W had an average of 1.03 scars per cell. Out of 100 cells counted, 103 bud scars were counted. The wild yeast BY4735 had an average of 2.46 bud scars per cell (100 cells counted, 246 bud scars). This indicates that the knocked-out gene significantly slowed down the reproduction rate of these cells.
_2_48.png)
picture does not include all cells that were counted and added for a visual of bud scars
