Difference between revisions of "YHR033W"

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===[[UW-Stout/Ethanol_FA22|Ethanol]]===
 
===[[UW-Stout/Ethanol_FA22|Ethanol]]===
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[[Image:Best Ethanol KO graph.png|Graph|center|700px|YJL133C-A]]
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YHR033W is slightly above YJL133C-A in terms of growth but has a similar curve. The doubling time is 76.63. Ethanol didn't affect the growth.
  
 
===[[UW-Stout/Caffeine_FA22|Caffeine]]===
 
===[[UW-Stout/Caffeine_FA22|Caffeine]]===
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[[Image:F_graph.png|100pxs|Control]]
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[[Image:Knockout_v._caffeine_trial1_Viv.png|100pxs|Trial 1]]
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[[Image:Knockout_gene_v._Caffeine_trial_2.png|600px|Trial 2]]
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====Interpretation====
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The average doubling time for the YHR033W (C5) gene in standard conditions was 87 minutes. Under caffeine stress, the average doubling time was 165.5 minutes. That is a 90.2% increase in doubling time which means that cell growth was negatively affected (slowed down) by this type of stress.
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===Hydrogen Peroxide===
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[[Image:2.jpg|center|thumb|600px|The x-axis is time in minutes and the y-axis is OD600]]
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This is the link to the protocol page: [[UW-Stout/Hydrogen Peroxide FA22]].
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The doubling time for the YHR033W gene when stressed is 375 minutes and without stress is 221 minutes. The BY4375 under stress had a 598 minute doubling time and with no stress there was a 229 minute doubling time. The YHR033W gene presented a doubling time that was much faster than the wild type yeast. This data demonstrates that the YHR033W gene was more resistant to the hydrogen peroxide than the wild type. There is also a chance this change to be due from experimental error such as not vortexing the wild type yeast or having other microorganisms on your gloves. When no stress was present, the wild type yeast and YHR003W had very similar doubling time.
  
 
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{{RefHelp}}
 
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==UW Stout/FA 2022 Glycerol Media==
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===Introduction===
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Following the [[UW-Stout/Glycerol FA22]] protocol, 5 knockout strains of yeast cell were tortured within a glycerol media solution.
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===Results===
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[[Image:Knockout_4_Trial_1.png|600px]]
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[[Image:Negative_Control_KO4_Trial_1.png|600px]]
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[[Image:Knockout_4_Trial_2.png|600px]]
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[[Image:Negative_Control_KO4_Trial_2.png|600px]]
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[[Image:Growth_Curve_KO4_Trial_3.png|600px]]
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[[Image:Negative_Control_KO4_Trial_3.png|600px]]
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The following doubling times were calculated:
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* Glycerol Trial 1: NA
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* Negative Control Trial 1: 199 minutes
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* Glycerol Trial 2: 526 minutes
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* Negative Control Trial 2: 215 minutes
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* Glycerol Trial 3:2531 minutes
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* Negative Control Trial 3: 277 minutes
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===Analysis/Conclusion===
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==UW Stout/FA 2022 Bud Scars==
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Cells that had the gene YHR033W had an average of 1.03 scars per cell. Out of 100 cells counted, 103 bud scars were counted. The wild yeast BY4735 had an average of 2.46 bud scars per cell (100 cells counted, 246 bud scars). This indicates that the knocked-out gene significantly slowed down the reproduction rate of these cells.
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[[File:Yeast_4(2)_2_48.png]]
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''picture does not include all cells that were counted and added for a visual of bud scars''

Latest revision as of 17:45, 20 December 2022

Share your knowledge...Edit this entry! <protect>

Systematic name YHR033W
Gene name
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr VIII:175548..176819
Primary SGDID S000001075


Description of YHR033W: Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm[1]




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Community Commentary

About Community Commentary. Please share your knowledge!

Ethanol

YJL133C-A

YHR033W is slightly above YJL133C-A in terms of growth but has a similar curve. The doubling time is 76.63. Ethanol didn't affect the growth.

Caffeine

Control

Trial 1

Trial 2

Interpretation

The average doubling time for the YHR033W (C5) gene in standard conditions was 87 minutes. Under caffeine stress, the average doubling time was 165.5 minutes. That is a 90.2% increase in doubling time which means that cell growth was negatively affected (slowed down) by this type of stress.

Hydrogen Peroxide

The x-axis is time in minutes and the y-axis is OD600

This is the link to the protocol page: UW-Stout/Hydrogen Peroxide FA22.

The doubling time for the YHR033W gene when stressed is 375 minutes and without stress is 221 minutes. The BY4375 under stress had a 598 minute doubling time and with no stress there was a 229 minute doubling time. The YHR033W gene presented a doubling time that was much faster than the wild type yeast. This data demonstrates that the YHR033W gene was more resistant to the hydrogen peroxide than the wild type. There is also a chance this change to be due from experimental error such as not vortexing the wild type yeast or having other microorganisms on your gloves. When no stress was present, the wild type yeast and YHR003W had very similar doubling time.



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References

See Help:References on how to add references

  1. ↑ Kumar A, et al. (2002) Subcellular localization of the yeast proteome. Genes Dev 16(6):707-19 SGD PMID 11914276

See Help:Categories on how to add the wiki page for this gene to a Category </protect>

UW Stout/FA 2022 Glycerol Media

Introduction

Following the UW-Stout/Glycerol FA22 protocol, 5 knockout strains of yeast cell were tortured within a glycerol media solution.

Results

Knockout 4 Trial 1.png Negative Control KO4 Trial 1.png Knockout 4 Trial 2.png Negative Control KO4 Trial 2.png Growth Curve KO4 Trial 3.png Negative Control KO4 Trial 3.png

The following doubling times were calculated:

  • Glycerol Trial 1: NA
  • Negative Control Trial 1: 199 minutes
  • Glycerol Trial 2: 526 minutes
  • Negative Control Trial 2: 215 minutes
  • Glycerol Trial 3:2531 minutes
  • Negative Control Trial 3: 277 minutes

Analysis/Conclusion

UW Stout/FA 2022 Bud Scars

Cells that had the gene YHR033W had an average of 1.03 scars per cell. Out of 100 cells counted, 103 bud scars were counted. The wild yeast BY4735 had an average of 2.46 bud scars per cell (100 cells counted, 246 bud scars). This indicates that the knocked-out gene significantly slowed down the reproduction rate of these cells. Yeast 4(2) 2 48.png picture does not include all cells that were counted and added for a visual of bud scars

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