Difference between revisions of "YHR033W"

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(Ethanol)
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The average doubling time for the YHR033W (C5) gene in standard conditions was 87 minutes. Under caffeine stress, the average doubling time was 165.5 minutes. That is a 90.2% increase in doubling time which means that cell growth was negatively affected (slowed down) by this type of stress.  
 
The average doubling time for the YHR033W (C5) gene in standard conditions was 87 minutes. Under caffeine stress, the average doubling time was 165.5 minutes. That is a 90.2% increase in doubling time which means that cell growth was negatively affected (slowed down) by this type of stress.  
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===Hydrogen Peroxide===
  
 
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Revision as of 14:01, 19 December 2022

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Systematic name YHR033W
Gene name
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr VIII:175548..176819
Primary SGDID S000001075


Description of YHR033W: Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm[1]




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Community Commentary

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Ethanol

YJL133C-A

YHR033W is slightly above YJL133C-A in terms of growth but has a similar curve. The doubling time is 76.63. Ethanol didn't affect the growth.

Caffeine

Control

Trial 1

Trial 2

Interpretation

The average doubling time for the YHR033W (C5) gene in standard conditions was 87 minutes. Under caffeine stress, the average doubling time was 165.5 minutes. That is a 90.2% increase in doubling time which means that cell growth was negatively affected (slowed down) by this type of stress.

Hydrogen Peroxide

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References

See Help:References on how to add references

  1. Kumar A, et al. (2002) Subcellular localization of the yeast proteome. Genes Dev 16(6):707-19 SGD PMID 11914276

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UW Stout/FA 2022 Glycerol Media

Introduction

Following the UW-Stout/Glycerol FA22 protocol, 5 knockout strains of yeast cell were tortured within a glycerol media solution.

Results

Knockout 4 Trial 1.png Negative Control KO4 Trial 1.png Knockout 4 Trial 2.png Negative Control KO4 Trial 2.png Growth Curve KO4 Trial 3.png Negative Control KO4 Trial 3.png

The following doubling times were calculated:

  • Glycerol Trial 1: NA
  • Negative Control Trial 1: 199 minutes
  • Glycerol Trial 2: 526 minutes
  • Negative Control Trial 2: 215 minutes
  • Glycerol Trial 3:2531 minutes
  • Negative Control Trial 3: 277 minutes

Analysis/Conclusion