Difference between revisions of "YHR033W"

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(UW Stout/FA 2022 Glycerol Media)
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Following the [[UW-Stout/Glycerol FA22]] protocol, 5 knockout strains of yeast cell were tortured within a glycerol media solution.
 
Following the [[UW-Stout/Glycerol FA22]] protocol, 5 knockout strains of yeast cell were tortured within a glycerol media solution.
 
===Results===
 
===Results===
 +
Trial 3 glycerol Doubling time in glycerol: 2531
 +
Trial 2:glyercol Doubling time;526min
 +
Negative Control 1: 199min
 +
Negative Control 2: 215min
 +
Negative Control 3: 277min
 +
Trial 2:glyercol Doubling time;526min
 
===Analysis/Conclusion===
 
===Analysis/Conclusion===

Revision as of 16:33, 16 December 2022

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Systematic name YHR033W
Gene name
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr VIII:175548..176819
Primary SGDID S000001075


Description of YHR033W: Putative protein of unknown function; epitope-tagged protein localizes to the cytoplasm[1]




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Community Commentary

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Ethanol

Caffeine

Control

Trial 1

Trial 2

Interpretation

The average doubling time for the YHR033W (C5) gene in standard conditions was 87 minutes. Under caffeine stress, the average doubling time was 165.5 minutes. That is a 90.2% increase in doubling time which means that cell growth was negatively affected (slowed down) by this type of stress.



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References

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  1. Kumar A, et al. (2002) Subcellular localization of the yeast proteome. Genes Dev 16(6):707-19 SGD PMID 11914276

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UW Stout/FA 2022 Glycerol Media

Introduction

Following the UW-Stout/Glycerol FA22 protocol, 5 knockout strains of yeast cell were tortured within a glycerol media solution.

Results

Trial 3 glycerol Doubling time in glycerol: 2531 Trial 2:glyercol Doubling time;526min Negative Control 1: 199min Negative Control 2: 215min Negative Control 3: 277min Trial 2:glyercol Doubling time;526min

Analysis/Conclusion