YGL140C

From SGD-Wiki
Revision as of 11:40, 12 December 2019 by Teagueb (talk | contribs) (Community Commentary)
Jump to: navigation, search

Share your knowledge...Edit this entry! <protect>

Systematic name YGL140C
Gene name
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr VII:245012..241353
Primary SGDID S000003108


Description of YGL140C: Putative protein of unknown function; non-essential gene; contains multiple predicted transmembrane domains[1][2][3]




</protect>

Community Commentary

About Community Commentary. Please share your knowledge!

This gene is part of the UW-Stout Orphan Gene Project. Learn more here.

Growth Curve

YGL140C YPD.png

In a BY4735 background, knocking out YGL140C seems to have little or no effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 414 minutes, while the YGL140C knock-out strain's doubling time was 432 minutes. (These doubling times are the means of three experiments.)

Caffeine and Yeast Cells

megan27.png

This graph shows the growth curve with and without caffeine. It has the knockout strain and wild type yeast cells. The caffeine enhanced the growth in both wild type and knockout strain. The average doubling time between the three experiments for the knockout strand was 777.97 minutes. The caffeine also had a positive effect on the growth curve for the wild type with the average doubling time being 1245.02 minutes. The results were concluded from the wild type and knockout strand was with this amount of caffeine it leads to an increase in growth.

The protocol can be found at

Caffeine

yGL140cUV Exposure.png

This bar graph shows that compared to a BY4735 background' Knocking out yGL140c have no effect on how sensitive yeast is to UV Light

The protocol can be found at

UV Light Exposure

YGL140C ethonal.png



<protect>

References

See Help:References on how to add references

  1. Giaever G, et al. (2002) Functional profiling of the Saccharomyces cerevisiae genome. Nature 418(6896):387-91 SGD PMID 12140549
  2. Voet M, et al. (1997) The sequence of a nearly unclonable 22.8 kb segment on the left arm chromosome VII from Saccharomyces cerevisiae reveals ARO2, RPL9A, TIP1, MRF1 genes and six new open reading frames. Yeast 13(2):177-82 SGD PMID 9046099
  3. Volckaert G, et al. (2003) Disruption of 12 ORFs located on chromosomes IV, VII and XIV of Saccharomyces cerevisiae reveals two essential genes. Yeast 20(1):79-88 SGD PMID 12489128

See Help:Categories on how to add the wiki page for this gene to a Category </protect>