YFL064C

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Systematic name YFL064C
Gene name
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr VI:3846..3322
Primary SGDID S000001830


Description of YFL064C: Putative protein of unknown function[1]




Community Commentary

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This gene is part of the UW-Stout Orphan Gene Project. Learn more here.

UW-Stout/Sensitivity To Nitrogen Starvation

Growth of YFL064C on synthetic defined media (SDC).
Growth of YFL064C on omission media that lacks nitrogen.
Growth of transferred BY4735 on Synthetic defined media.

Knocking out the YFL064C seems to have no effect on growth after incubating for 5 days on the Nitrogen omitted media.


Ultraviolet Sensitivity

YFL064C uv light.jpg BY4735(wild) uv light.jpg

YFL064C is more sensitive than BY4735(wild) given that there is less colonizes present when under the same stress.

Growth Curve

yfl064c growth.png

In a BY4735 background, knocking out YFL064C seems to have no effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 124 minutes, and the YFL064C knock-out strain's doubling time was also 124 minutes. However, the knock-out strain did reach a substantially higher OD600 before saturating.

image007.png

Methanol Sensitivity

The wild type had a doubling time of 200 min, the YFL064c had a doubling time of 170 min. The methanol had little to no effect on these strains.

[1]

YFL064C sensitivity Methanol.png

Stressing with Hydroxyurea

Hydroxyurea Protocol -The wild type strain had only a small difference in doubling time when stressed with Hydroxyurea;YFL064C had about a 10 minute difference of doubling time when stressed with hydroxyurea, indicating that this gene strain most likely does not play a vital role in DNA replication.

HU YFL064C.png


References

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  1. SGD (2006) No information available SGD PMID

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