YER186C
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| Systematic name | YER186C |
| Gene name | |
| Aliases | |
| Feature type | ORF, Uncharacterized |
| Coordinates | Chr V:562625..561705 |
| Primary SGDID | S000000988 |
Description of YER186C: Putative protein of unknown function[1]
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Contents
Community Commentary
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DNA and RNA Details
Other DNA and RNA Details
Other Topic: expression
Specifically higher expression in phosphorus limited chemostat cultures versus phosphorus excess. [2] [3]
Caffeine
Interpretation
The average doubling time for the YEr186C (C3) gene in standard conditions was 87 minutes. Under caffeine stress, the average doubling time was 181 minutes. That is a 108% increase in doubling time which means that cell growth was negatively affected (slowed down) by this type of stress.
Ethanol
YER186C is our outlier for this Ethanol experiment. The Ethanol dramatically decreased cell growth. The doubling time is 189.60
Hydrogen Peroxide
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References
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- ↑ SGD (2006) No information available SGD PMID
- ↑ Boer VM, et al. (2003) The genome-wide transcriptional responses of Saccharomyces cerevisiae grown on glucose in aerobic chemostat cultures limited for carbon, nitrogen, phosphorus, or sulfur. J Biol Chem 278(5):3265-74 SGD PMID 12414795
- ↑ submitted by Viktor Boer on 2003-07-25
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UW Stout/FA 2022 Glycerol Media
Introduction
Following the UW-Stout/Glycerol FA22 protocol, 5 knockout strains of yeast cell were tortured within a glycerol media solution.
Results
The following doubling times were calculated:
- Glycerol Trial 1: NA
- Negative Control Trial 1: 173min
- Glycerol Trial 2: NA
- Negative Control Trial 2: 287min
- Glycerol Trial 3: NA
- Negative Control Trial 3: 262min
Analysis/Conclusion
The results from glycerol torture on the YER186C knockout were quite interesting. All 3 graphs looked ridiculous, but there is one point that is clear: the yeast cells did not grow very successfully in the glycerol media without this gene. Trial 1's data was all around a little scattered and the jump near the end was a bit concerning considering it happened in every single experiment- so we weren't very confident in the results. However, in Trial 2 it continued to be weird... The OD600 reading did not seem to go up at all! This could mean the cells are all dead or not reproducing. This is intriguing data! The negative control graph for this trial even looked normal which leads us to believe that the flatlined data wasn't just a slip up. Trial 3, like trial 1, also seemed like a bust. It was interesting that the data showed a significant decrease in the original cells, but one thing is for certain: the yeast was definitely not reproducing and was most likely dead.
