YER076C

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Systematic name YER076C
Gene name
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr V:313498..312590
Primary SGDID S000000878


Description of YER076C: Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization[1][2][3]




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Community Commentary

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Ethanol

Caffeine

Control

Trial 1

Trial 2

Interpretation

The average doubling time for the YER076C (C4) gene in standard conditions was 87.5 minutes. Under caffeine stress, the average doubling time was 139.5 minutes. That is a 59.4% increase in doubling time which means that cell growth was negatively affected (slowed down) by this type of stress.




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References

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  1. Reinders J, et al. (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54 SGD PMID 16823961
  2. Sickmann A, et al. (2003) The proteome of Saccharomyces cerevisiae mitochondria. Proc Natl Acad Sci U S A 100(23):13207-12 SGD PMID 14576278
  3. Terashima H, et al. (2002) Sequence-based approach for identification of cell wall proteins in Saccharomyces cerevisiae. Curr Genet 40(5):311-6 SGD PMID 11935221

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UW Stout/FA 2022 Glycerol Media

Introduction

Following the UW-Stout/Glycerol FA22 protocol, 5 knockout strains of yeast cell were tortured within a glycerol media solution.

Results

Knockout 3 Trial 1.png Negative Control KO3 Trial 1.png Knockout 3 Trial 2.png Negative Control KO3 Trial 2.png Growth Curve KO3 Trial 3.png Negative Control KO3 Trial 3.png

The following doubling times were calculated:

  • Glycerol Trial 1: NA
  • Negative Control Trial 1: 188 min
  • Glycerol Trial 2: 144 min
  • Negative Control Trial 2: 192 min
  • Glycerol Trial 3:2531 min
  • Negative Control Trial 3: 212 min

Analysis/Conclusion