Difference between revisions of "YER076C"

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(Results)
(Results)
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===Results===
 
===Results===
 
[[Image:Knockout_3_Trial_1.png|600px]]
 
[[Image:Knockout_3_Trial_1.png|600px]]
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[[Image:Glycerol_Negative_Control_KO3_Trial_1.png|600px]]
  
 
===Analysis/Conclusion===
 
===Analysis/Conclusion===

Revision as of 16:16, 16 December 2022

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Systematic name YER076C
Gene name
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr V:313498..312590
Primary SGDID S000000878


Description of YER076C: Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; analysis of HA-tagged protein suggests a membrane localization[1][2][3]




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Community Commentary

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Ethanol

Caffeine

Control

Trial 1

Trial 2

Interpretation

The average doubling time for the YER076C (C4) gene in standard conditions was 87.5 minutes. Under caffeine stress, the average doubling time was 139.5 minutes. That is a 59.4% increase in doubling time which means that cell growth was negatively affected (slowed down) by this type of stress.




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References

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  1. Reinders J, et al. (2006) Toward the complete yeast mitochondrial proteome: multidimensional separation techniques for mitochondrial proteomics. J Proteome Res 5(7):1543-54 SGD PMID 16823961
  2. Sickmann A, et al. (2003) The proteome of Saccharomyces cerevisiae mitochondria. Proc Natl Acad Sci U S A 100(23):13207-12 SGD PMID 14576278
  3. Terashima H, et al. (2002) Sequence-based approach for identification of cell wall proteins in Saccharomyces cerevisiae. Curr Genet 40(5):311-6 SGD PMID 11935221

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UW Stout/FA 2022 Glycerol Media

Introduction

Following the UW-Stout/Glycerol FA22 protocol, 5 knockout strains of yeast cell were tortured within a glycerol media solution.

Results

Knockout 3 Trial 1.png 600px

Analysis/Conclusion