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Systematic name YEL035C
Gene name UTR5
Feature type ORF, Uncharacterized
Coordinates Chr V:85545..85045
Primary SGDID S000000761

Description of YEL035C: Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions[1][2]

Community Commentary

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This gene is part of the UW-Stout Orphan Gene Project. Learn more here.

UW-Stout/Sensitivity To Nitrogen Starvation

Growth of YEL035C on synthetic defined media (SDC).
Growth of YELO35C on omission media that lacks nitrogen.
Growth of transferred BY4735 on Synthetic defined media.

Knocking out the YGL035C seems to have no effect on growth after incubating for 5 days on the Nitrogen omitted media.

Ultraviolet Sensitivity

YEL035C uv light.jpg BY4735(wild) uv light.jpg

YEL035C is more sensitive than BY4735(wild) given that there is less colonizes present when under the same stress.

Growth Rate in YPD

yel035c growth.png

In a BY4735 background, knocking out YEL035C seems to have a moderate effect on growth rate in log-phase in YPD media. In this assay, the BY4735 strain's doubling time was 124 minutes, while the YEL035C knock-out strain's doubling time was 220 minutes.

G-418 Stress


Methanol sensitivity

The wild type strain had a little difference in the doubling time when we added methanol, but the methanol killed all of the cells in the YEL035c strain. [[1]]


Stressing with Hydroxyurea

Hydroxyurea Protocol - The wild type strain had only a small difference in doubling time when stressed with Hydroxyurea; however, the doubling time of the YEL035C strain was much higher, indicating that the gene is more sensitive to the stress and that this gene probably plays a role in DNA replication.



See Help:References on how to add references

  1. Lopez MC and Baker HV (2000) Understanding the growth phenotype of the yeast gcr1 mutant in terms of global genomic expression patterns. J Bacteriol 182(17):4970-8 SGD PMID 10940042
  2. Snoek IS and Steensma HY (2006) Why does Kluyveromyces lactis not grow under anaerobic conditions? Comparison of essential anaerobic genes of Saccharomyces cerevisiae with the Kluyveromyces lactis genome. FEMS Yeast Res 6(3):393-403 SGD PMID 16630279

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