Difference between revisions of "YEL035C"

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(Methanol sensitivity)
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===[[UW-Stout/Ultraviolet Light|Ultraviolet Sensitivity]]===
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[[File:YEL035C uv light.jpeg|300 px]]
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[[File:BY4735(wild) uv light.jpg|300 px]]
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YEL035C is more sensitive than BY4735(wild) given that there is less colonizes present when under the same stress.
  
 
===[[UW-Stout/Growth_Curve|Growth Rate in YPD]]===
 
===[[UW-Stout/Growth_Curve|Growth Rate in YPD]]===

Revision as of 07:13, 2 May 2019

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Systematic name YEL035C
Gene name UTR5
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr V:85545..85045
Primary SGDID S000000761


Description of YEL035C: Protein of unknown function; transcription may be regulated by Gcr1p; essential for growth under standard (aerobic) conditions but not under anaerobic conditions[1][2]




Community Commentary

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This gene is part of the UW-Stout Orphan Gene Project. Learn more here.

Ultraviolet Sensitivity

300 px BY4735(wild) uv light.jpg

YEL035C is more sensitive than BY4735(wild) given that there is less colonizes present when under the same stress.

Growth Rate in YPD

yel035c growth.png

In a BY4735 background, knocking out YEL035C seems to have a moderate effect on growth rate in log-phase in YPD media. In this assay, the BY4735 strain's doubling time was 124 minutes, while the YEL035C knock-out strain's doubling time was 220 minutes.

G-418 Stress

image016.png


Methanol sensitivity

The wild type strain had a little difference in the doubling time when we added methanol, but the methanol killed all of the cells in the YEL035c strain. [[1]]

YEL035C.png


References

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  1. Lopez MC and Baker HV (2000) Understanding the growth phenotype of the yeast gcr1 mutant in terms of global genomic expression patterns. J Bacteriol 182(17):4970-8 SGD PMID 10940042
  2. Snoek IS and Steensma HY (2006) Why does Kluyveromyces lactis not grow under anaerobic conditions? Comparison of essential anaerobic genes of Saccharomyces cerevisiae with the Kluyveromyces lactis genome. FEMS Yeast Res 6(3):393-403 SGD PMID 16630279

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