Difference between revisions of "YEL021W"
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| − | |valign="top" nowrap bgcolor="{{SGDblue}}"| '''Systematic name''' || [http:// | + | |valign="top" nowrap bgcolor="{{SGDblue}}"| '''Systematic name''' || [http://www.yeastgenome.org/cgi-bin/locus.pl?dbid=S000000747 YEL021W] |
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|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Gene name''' ||''URA3 '' | |valign="top" nowrap bgcolor="{{SGDblue}}"| '''Gene name''' ||''URA3 '' | ||
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|valign="top" nowrap bgcolor="{{SGDblue}}"| '''Coordinates''' | |valign="top" nowrap bgcolor="{{SGDblue}}"| '''Coordinates''' | ||
|nowrap| Chr V:116167..116970 | |nowrap| Chr V:116167..116970 | ||
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| + | |valign="top" nowrap bgcolor="{{SGDblue}}"| '''Primary SGDID''' || S000000747 | ||
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| − | '''Description of | + | '''Description of YEL021W:''' Orotidine-5'-phosphate (OMP) decarboxylase, catalyzes the sixth enzymatic step in the de novo biosynthesis of pyrimidines, converting OMP into uridine monophosphate (UMP); converts 5-FOA into 5-fluorouracil, a toxic compound<ref name='S000046487'>Alani E and Kleckner N (1987) A new type of fusion analysis applicable to many organisms: protein fusions to the URA3 gene of yeast. Genetics 117(1):5-12 {{SGDpaper|S000046487}} PMID 3311876</ref><ref name='S000051375'>Boeke JD, et al. (1984) A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance. Mol Gen Genet 197(2):345-6 {{SGDpaper|S000051375}} PMID 6394957</ref><ref name='S000053190'>Boeke JD, et al. (1987) 5-Fluoroorotic acid as a selective agent in yeast molecular genetics. Methods Enzymol 154:164-75 {{SGDpaper|S000053190}} PMID 3323810</ref><ref name='S000056535'>Jones ME (1992) Orotidylate decarboxylase of yeast and man. Curr Top Cell Regul 33:331-42 {{SGDpaper|S000056535}} PMID 1499340</ref><ref name='S000065623'>Lacroute F (1968) Regulation of pyrimidine biosynthesis in Saccharomyces cerevisiae. J Bacteriol 95(3):824-32 {{SGDpaper|S000065623}} PMID 5651325</ref><ref name='S000081430'>Umezu K, et al. (1971) Purification and properties of orotidine-5'-phosphate pyrophosphorylase and orotidine-5'-phosphate decarboxylase from baker's yeast. J Biochem 70(2):249-62 |
| − | {{SGDpaper| | + | {{SGDpaper|S000081430}} PMID 5094209</ref> |
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==Community Commentary== | ==Community Commentary== | ||
{{CommentaryHelp}} | {{CommentaryHelp}} | ||
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| + | <!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation --> | ||
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| + | Specifically higher expression in carbon limited chemostat cultures versus carbon excess. | ||
| + | <ref>Boer VM, et al. (2003) The genome-wide transcriptional responses of Saccharomyces cerevisiae grown on glucose in aerobic chemostat cultures limited for carbon, nitrogen, phosphorus, or sulfur. | ||
| + | J Biol Chem 278(5):3265-74</ref> | ||
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Latest revision as of 07:45, 23 January 2012
Share your knowledge...Edit this entry! <protect>
| Systematic name | YEL021W |
| Gene name | URA3 |
| Aliases | |
| Feature type | ORF, Verified |
| Coordinates | Chr V:116167..116970 |
| Primary SGDID | S000000747 |
Description of YEL021W: Orotidine-5'-phosphate (OMP) decarboxylase, catalyzes the sixth enzymatic step in the de novo biosynthesis of pyrimidines, converting OMP into uridine monophosphate (UMP); converts 5-FOA into 5-fluorouracil, a toxic compound[1][2][3][4][5][6]
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Contents
Community Commentary
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References
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- ↑ Alani E and Kleckner N (1987) A new type of fusion analysis applicable to many organisms: protein fusions to the URA3 gene of yeast. Genetics 117(1):5-12 SGD PMID 3311876
- ↑ Boeke JD, et al. (1984) A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance. Mol Gen Genet 197(2):345-6 SGD PMID 6394957
- ↑ Boeke JD, et al. (1987) 5-Fluoroorotic acid as a selective agent in yeast molecular genetics. Methods Enzymol 154:164-75 SGD PMID 3323810
- ↑ Jones ME (1992) Orotidylate decarboxylase of yeast and man. Curr Top Cell Regul 33:331-42 SGD PMID 1499340
- ↑ Lacroute F (1968) Regulation of pyrimidine biosynthesis in Saccharomyces cerevisiae. J Bacteriol 95(3):824-32 SGD PMID 5651325
- ↑ Umezu K, et al. (1971) Purification and properties of orotidine-5'-phosphate pyrophosphorylase and orotidine-5'-phosphate decarboxylase from baker's yeast. J Biochem 70(2):249-62 SGD PMID 5094209
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