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|Feature type||ORF, Uncharacterized|
Description of YCR100C: Putative protein of unknown function
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This gene is part of the UW-Stout Orphan Gene Project. Learn more here.
Knocking out the YCR100C seems to have no effect on growth after incubating for 5 days on the Nitrogen omitted media.
In a BY4735 background, knocking out YCR100C seems to have a moderate effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 124 minutes, while the YCR100C knock-out strain's doubling time was 219 minutes.
YCR100C is more sensitive than BY4735(wild) given that there is less colonizes present when under the same stress.
The wild type had a doubling time of 150 min, the YCR100c had a doubling time of 350 min. The methanol slowed the yeast down and that means this strain is more sensitive.
Stressing with Hydroxyurea
Hydroxyurea Protocl -The wild type strain had only a small difference in doubling time when stressed with Hydroxyurea;YCR100C showed about a 20 minute difference in doubling time when stressed with hydroxyurea, indicating that this gene most likely does not play a vital role in DNA replication.
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- SGD (2006) No information available SGD PMID
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