Difference between revisions of "YCL063W"

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Specifically higher expression in carbon limited chemostat cultures versus carbon excess.
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<ref>Boer VM, et al. (2003) The genome-wide transcriptional responses of Saccharomyces cerevisiae grown on glucose in aerobic chemostat cultures limited for carbon, nitrogen, phosphorus, or sulfur.
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J Biol Chem 278(5):3265-74</ref>
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Revision as of 13:02, 21 February 2007

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Systematic name YCL063W
Gene name VAC17
Aliases YCL062W
Feature type ORF, Verified
Coordinates Chr III:17290..18561


Description of YCL063W: Protein involved in vacuole inheritance; acts as a vacuole-specific receptor for myosin Myo2p[1][2]




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Community Commentary

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Alleles, Strains, and Phenotypes

Partial Deletion

Strain Background: LWY 7235
Genotype: LWY 5798 (vac17::TRP1)
Phenotype(s): Loss of function (Null), Recessive, Viable Mutation type(s): Partial deletion from 17364 to partialDelTo

In LWY5798, residues 26-357 of Vac17p was substituted with the TRP1 gene. [2] [3]

Interactions

Physical

Physical interaction with VAC8
Immunoprecipitating Vac17p with sheep anti-Vac17p antibody coprecipitates Vac8p. [2] [3]

Physical interaction with MYO2
Immunoprecipitating Vac17p with sheep anti-Vac17p antibody coprecipitates Myo2p. [2] [3]

Two Hybrid

Two Hybrid interaction with VAC8
Residues 290-380 of Vac17p interact with Vac8p in a yeast two-hybrid analysis. [2] [3]

Protein Details

Protein Degradation

Vac17p contains a PEST sequence, a rapid degradation signal. The PEST-dependent degradation appears to occur in the bud after vacuoles arrive in the bud. [2] [3]

Protein Function/Process

Vac17p is involved in vacuole inheritance. Deletion of VAC17 gene blocks vacuole inheritance. Vac17p functions as the vacuole-specific Myp2p receptor. It forms a complex with Myo2p and Vac8p, a vacuolar membrane protein. Vac17p connects Myo2p to Vac8p and thus the vacuole membrane. [2] [3]

Protein Localization

Vac17p is localized on the vacuole membrane in a Vac8p-dependent manner. [2] [3]



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References

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  1. ↑ Ishikawa K, et al. (2003) Identification of an organelle-specific myosin V receptor. J Cell Biol 160(6):887-97 SGD PMID 12642614
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 Tang F, et al. (2003) Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole. Nature 422(6927):87-92 SGD PMID 12594460 Cite error: Invalid <ref> tag; name "S000072665" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000072665" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000072665" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000072665" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000072665" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000072665" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000072665" defined multiple times with different content
  3. ↑ 3.0 3.1 3.2 3.3 3.4 3.5 3.6 submitted by Fusheng Tang on 2003-03-17

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