Difference between revisions of "YCL063W"
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Revision as of 08:17, 30 January 2007
Share your knowledge...Edit this entry! <protect>
| Systematic name | YCL063W |
| Gene name | VAC17 |
| Aliases | YCL062W |
| Feature type | ORF, Verified |
| Coordinates | Chr III:17290..18561 |
Description of YCL063W: Protein involved in vacuole inheritance; acts as a vacuole-specific receptor for myosin Myo2p[1][2]
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Contents
Community Commentary
About Community Commentary. Please share your knowledge!
Alleles, Strains, and Phenotypes
Partial Deletion
Strain Background: LWY 7235
Genotype: LWY 5798 (vac17::TRP1)
Phenotype(s): Loss of function (Null), Recessive, Viable
Mutation type(s): Partial deletion from 17364 to partialDelTo
In LWY5798, residues 26-357 of Vac17p was substituted with the TRP1 gene. [2] [3]
Interactions
Physical
Physical interaction with VAC8
Immunoprecipitating Vac17p with sheep anti-Vac17p antibody coprecipitates Vac8p. [2] [3]
Physical interaction with MYO2
Immunoprecipitating Vac17p with sheep anti-Vac17p antibody coprecipitates Myo2p. [2] [3]
Two Hybrid
Two Hybrid interaction with VAC8
Residues 290-380 of Vac17p interact with Vac8p in a yeast two-hybrid analysis. [2] [3]
Protein Details
Protein Degradation
Vac17p contains a PEST sequence, a rapid degradation signal. The PEST-dependent degradation appears to occur in the bud after vacuoles arrive in the bud. [2] [3]
Protein Function/Process
Vac17p is involved in vacuole inheritance. Deletion of VAC17 gene blocks vacuole inheritance. Vac17p functions as the vacuole-specific Myp2p receptor. It forms a complex with Myo2p and Vac8p, a vacuolar membrane protein. Vac17p connects Myo2p to Vac8p and thus the vacuole membrane. [2] [3]
Protein Localization
Vac17p is localized on the vacuole membrane in a Vac8p-dependent manner. [2] [3]
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References
See Help:References on how to add references
- ↑ Ishikawa K, et al. (2003) Identification of an organelle-specific myosin V receptor. J Cell Biol 160(6):887-97 SGD PMID 12642614
- ↑ 2.0 2.1 2.2 2.3 2.4 2.5 2.6 2.7 Tang F, et al. (2003) Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole. Nature 422(6927):87-92
SGD PMID 12594460 Cite error: Invalid
<ref>tag; name "S000072665" defined multiple times with different content Cite error: Invalid<ref>tag; name "S000072665" defined multiple times with different content Cite error: Invalid<ref>tag; name "S000072665" defined multiple times with different content Cite error: Invalid<ref>tag; name "S000072665" defined multiple times with different content Cite error: Invalid<ref>tag; name "S000072665" defined multiple times with different content Cite error: Invalid<ref>tag; name "S000072665" defined multiple times with different content Cite error: Invalid<ref>tag; name "S000072665" defined multiple times with different content - ↑ 3.0 3.1 3.2 3.3 3.4 3.5 3.6 submitted by Fusheng Tang on 2003-03-17
See Help:Categories on how to add the wiki page for this gene to a Category </protect>
- Pages with reference errors
- ORF
- ORF, Verified
- Topic:Alleles, Strains, and Phenotypes
- Topic:Alleles, Strains, and Phenotypes:Partial Deletion
- Phenotype:Loss of function (Null)
- Phenotype:Recessive
- Phenotype:Viable
- Topic:Interactions
- Topic:Interactions:Physical
- Physical Interactions with VAC8
- Physical Interactions with MYO2
- Topic:Interactions:Two Hybrid
- Two Hybrid Interactions with VAC8
- Topic:Protein Details
- Topic:Protein Details:Protein Degradation
- Topic:Protein Details:Protein Function/Process
- Topic:Protein Details:Protein Localization
