Difference between revisions of "YCL063W"

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=== Alleles, Strains, and Phenotypes ===
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[[Category:Topic:Alleles, Strains, and Phenotypes]]
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==== Partial Deletion ====
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[[Category:Topic:Alleles, Strains, and Phenotypes:Partial Deletion]]
  
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'''Strain Background''': LWY 7235<br>
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'''Genotype''': LWY 5798 (vac17::TRP1)<br>
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'''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]], Recessive [[Category:Phenotype:Recessive]], Viable [[Category:Phenotype:Viable]]
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'''Mutation type(s)''': Partial deletion from 17364 to partialDelTo
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In LWY5798, residues 26-357 of Vac17p was substituted with the TRP1 gene. <ref name='S000072665'>Tang F, et al. (2003) Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole. Nature 422(6927):87-92 {{SGDpaper|S000072665}} PMID 12594460</ref> <ref name = 'CAset5804-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=5804 Fusheng Tang] on 2003-03-17</ref>
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{{ShortCenteredHR}}
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=== Interactions ===
 +
[[Category:Topic:Interactions]]
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==== Physical ====
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[[Category:Topic:Interactions:Physical]]
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Physical interaction with VAC8 [[Category:Physical Interactions with VAC8]]<br>
 +
Immunoprecipitating Vac17p with sheep anti-Vac17p antibody coprecipitates Vac8p. <ref name='S000072665'>Tang F, et al. (2003) Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole. Nature 422(6927):87-92 {{SGDpaper|S000072665}} PMID 12594460</ref> <ref name = 'CAset5804-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=5804 Fusheng Tang] on 2003-03-17</ref>
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{{ShortCenteredHR}}
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Physical interaction with MYO2 [[Category:Physical Interactions with MYO2]]<br>
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Immunoprecipitating Vac17p with sheep anti-Vac17p antibody coprecipitates Myo2p. <ref name='S000072665'>Tang F, et al. (2003) Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole. Nature 422(6927):87-92 {{SGDpaper|S000072665}} PMID 12594460</ref> <ref name = 'CAset5804-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=5804 Fusheng Tang] on 2003-03-17</ref>
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{{ShortCenteredHR}}
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==== Two Hybrid ====
 +
[[Category:Topic:Interactions:Two Hybrid]]
 +
Two Hybrid interaction with VAC8 [[Category:Two Hybrid Interactions with VAC8]]<br>
 +
Residues 290-380 of Vac17p interact with Vac8p in a yeast two-hybrid analysis. <ref name='S000072665'>Tang F, et al. (2003) Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole. Nature 422(6927):87-92 {{SGDpaper|S000072665}} PMID 12594460</ref> <ref name = 'CAset5804-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=5804 Fusheng Tang] on 2003-03-17</ref>
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{{ShortCenteredHR}}
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=== Protein Details ===
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[[Category:Topic:Protein Details]]
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==== Protein Degradation ====
 +
[[Category:Topic:Protein Details:Protein Degradation]]
 +
 +
Vac17p contains a PEST sequence, a rapid degradation signal. The PEST-dependent degradation appears to occur in the bud after vacuoles arrive in the bud. <ref name='S000072665'>Tang F, et al. (2003) Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole. Nature 422(6927):87-92 {{SGDpaper|S000072665}} PMID 12594460</ref> <ref name = 'CAset5804-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=5804 Fusheng Tang] on 2003-03-17</ref>
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{{ShortCenteredHR}}
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==== Protein Function/Process ====
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[[Category:Topic:Protein Details:Protein Function/Process]]
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Vac17p is involved in vacuole inheritance. Deletion of VAC17 gene blocks vacuole inheritance. Vac17p functions as the vacuole-specific Myp2p receptor. It forms a complex with Myo2p and Vac8p, a vacuolar membrane protein. Vac17p connects Myo2p to Vac8p and thus the vacuole membrane. <ref name='S000072665'>Tang F, et al. (2003) Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole. Nature 422(6927):87-92 {{SGDpaper|S000072665}} PMID 12594460</ref> <ref name = 'CAset5804-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=5804 Fusheng Tang] on 2003-03-17</ref>
 +
 +
{{ShortCenteredHR}}
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==== Protein Localization ====
 +
[[Category:Topic:Protein Details:Protein Localization]]
 +
 +
Vac17p is localized on the vacuole membrane in a Vac8p-dependent manner. <ref name='S000072665'>Tang F, et al. (2003) Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole. Nature 422(6927):87-92 {{SGDpaper|S000072665}} PMID 12594460</ref> <ref name = 'CAset5804-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=5804 Fusheng Tang] on 2003-03-17</ref>
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{{ShortCenteredHR}}
 
__TOC__
 
__TOC__
 
==Community Commentary==
 
==Community Commentary==
 
{{CommentaryHelp}}
 
{{CommentaryHelp}}
 +
=== Alleles, Strains, and Phenotypes ===
 +
[[Category:Topic:Alleles, Strains, and Phenotypes]]
 +
==== Partial Deletion ====
 +
[[Category:Topic:Alleles, Strains, and Phenotypes:Partial Deletion]]
 +
 +
'''Strain Background''': LWY 7235<br>
 +
'''Genotype''': LWY 5798 (vac17::TRP1)<br>
 +
'''Phenotype(s)''': Loss of function (Null) [[Category:Phenotype:Loss of function (Null)]], Recessive [[Category:Phenotype:Recessive]], Viable [[Category:Phenotype:Viable]]
 +
'''Mutation type(s)''': Partial deletion from 17364 to partialDelTo
 +
 +
In LWY5798, residues 26-357 of Vac17p was substituted with the TRP1 gene. <ref name='S000072665'>Tang F, et al. (2003) Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole. Nature 422(6927):87-92 {{SGDpaper|S000072665}} PMID 12594460</ref> <ref name = 'CAset5804-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=5804 Fusheng Tang] on 2003-03-17</ref>
 +
 +
{{ShortCenteredHR}}
 +
=== Interactions ===
 +
[[Category:Topic:Interactions]]
 +
==== Physical ====
 +
[[Category:Topic:Interactions:Physical]]
 +
Physical interaction with VAC8 [[Category:Physical Interactions with VAC8]]<br>
 +
Immunoprecipitating Vac17p with sheep anti-Vac17p antibody coprecipitates Vac8p. <ref name='S000072665'>Tang F, et al. (2003) Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole. Nature 422(6927):87-92 {{SGDpaper|S000072665}} PMID 12594460</ref> <ref name = 'CAset5804-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=5804 Fusheng Tang] on 2003-03-17</ref>
 +
 +
{{ShortCenteredHR}}
 +
Physical interaction with MYO2 [[Category:Physical Interactions with MYO2]]<br>
 +
Immunoprecipitating Vac17p with sheep anti-Vac17p antibody coprecipitates Myo2p. <ref name='S000072665'>Tang F, et al. (2003) Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole. Nature 422(6927):87-92 {{SGDpaper|S000072665}} PMID 12594460</ref> <ref name = 'CAset5804-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=5804 Fusheng Tang] on 2003-03-17</ref>
 +
 +
{{ShortCenteredHR}}
 +
==== Two Hybrid ====
 +
[[Category:Topic:Interactions:Two Hybrid]]
 +
Two Hybrid interaction with VAC8 [[Category:Two Hybrid Interactions with VAC8]]<br>
 +
Residues 290-380 of Vac17p interact with Vac8p in a yeast two-hybrid analysis. <ref name='S000072665'>Tang F, et al. (2003) Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole. Nature 422(6927):87-92 {{SGDpaper|S000072665}} PMID 12594460</ref> <ref name = 'CAset5804-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=5804 Fusheng Tang] on 2003-03-17</ref>
 +
 +
{{ShortCenteredHR}}
 +
=== Protein Details ===
 +
[[Category:Topic:Protein Details]]
 +
==== Protein Degradation ====
 +
[[Category:Topic:Protein Details:Protein Degradation]]
 +
 +
Vac17p contains a PEST sequence, a rapid degradation signal. The PEST-dependent degradation appears to occur in the bud after vacuoles arrive in the bud. <ref name='S000072665'>Tang F, et al. (2003) Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole. Nature 422(6927):87-92 {{SGDpaper|S000072665}} PMID 12594460</ref> <ref name = 'CAset5804-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=5804 Fusheng Tang] on 2003-03-17</ref>
 +
 +
{{ShortCenteredHR}}
 +
==== Protein Function/Process ====
 +
[[Category:Topic:Protein Details:Protein Function/Process]]
 +
 +
Vac17p is involved in vacuole inheritance. Deletion of VAC17 gene blocks vacuole inheritance. Vac17p functions as the vacuole-specific Myp2p receptor. It forms a complex with Myo2p and Vac8p, a vacuolar membrane protein. Vac17p connects Myo2p to Vac8p and thus the vacuole membrane. <ref name='S000072665'>Tang F, et al. (2003) Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole. Nature 422(6927):87-92 {{SGDpaper|S000072665}} PMID 12594460</ref> <ref name = 'CAset5804-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=5804 Fusheng Tang] on 2003-03-17</ref>
 +
 +
{{ShortCenteredHR}}
 +
==== Protein Localization ====
 +
[[Category:Topic:Protein Details:Protein Localization]]
 +
 +
Vac17p is localized on the vacuole membrane in a Vac8p-dependent manner. <ref name='S000072665'>Tang F, et al. (2003) Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole. Nature 422(6927):87-92 {{SGDpaper|S000072665}} PMID 12594460</ref> <ref name = 'CAset5804-2003-03-17'>submitted by [http://db.yeastgenome.org/cgi-bin/colleague/colleagueSearch?id=5804 Fusheng Tang] on 2003-03-17</ref>
  
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{{ShortCenteredHR}}
 
==References==
 
==References==
 
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Revision as of 17:05, 23 January 2007

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Systematic name YCL063W
Gene name VAC17
Aliases YCL062W
Feature type ORF, Verified
Coordinates Chr III:17290..18561
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Description of YCL063W: Protein involved in vacuole inheritance; acts as a vacuole-specific receptor for myosin Myo2p[1][2]



Alleles, Strains, and Phenotypes

Partial Deletion

Strain Background: LWY 7235
Genotype: LWY 5798 (vac17::TRP1)
Phenotype(s): Loss of function (Null), Recessive, Viable Mutation type(s): Partial deletion from 17364 to partialDelTo

In LWY5798, residues 26-357 of Vac17p was substituted with the TRP1 gene. [2] [3]


Interactions

Physical

Physical interaction with VAC8
Immunoprecipitating Vac17p with sheep anti-Vac17p antibody coprecipitates Vac8p. [2] [3]


Physical interaction with MYO2
Immunoprecipitating Vac17p with sheep anti-Vac17p antibody coprecipitates Myo2p. [2] [3]


Two Hybrid

Two Hybrid interaction with VAC8
Residues 290-380 of Vac17p interact with Vac8p in a yeast two-hybrid analysis. [2] [3]


Protein Details

Protein Degradation

Vac17p contains a PEST sequence, a rapid degradation signal. The PEST-dependent degradation appears to occur in the bud after vacuoles arrive in the bud. [2] [3]


Protein Function/Process

Vac17p is involved in vacuole inheritance. Deletion of VAC17 gene blocks vacuole inheritance. Vac17p functions as the vacuole-specific Myp2p receptor. It forms a complex with Myo2p and Vac8p, a vacuolar membrane protein. Vac17p connects Myo2p to Vac8p and thus the vacuole membrane. [2] [3]


Protein Localization

Vac17p is localized on the vacuole membrane in a Vac8p-dependent manner. [2] [3]


Community Commentary

About Community Commentary. Please share your knowledge!

Alleles, Strains, and Phenotypes

Partial Deletion

Strain Background: LWY 7235
Genotype: LWY 5798 (vac17::TRP1)
Phenotype(s): Loss of function (Null), Recessive, Viable Mutation type(s): Partial deletion from 17364 to partialDelTo

In LWY5798, residues 26-357 of Vac17p was substituted with the TRP1 gene. [2] [3]


Interactions

Physical

Physical interaction with VAC8
Immunoprecipitating Vac17p with sheep anti-Vac17p antibody coprecipitates Vac8p. [2] [3]


Physical interaction with MYO2
Immunoprecipitating Vac17p with sheep anti-Vac17p antibody coprecipitates Myo2p. [2] [3]


Two Hybrid

Two Hybrid interaction with VAC8
Residues 290-380 of Vac17p interact with Vac8p in a yeast two-hybrid analysis. [2] [3]


Protein Details

Protein Degradation

Vac17p contains a PEST sequence, a rapid degradation signal. The PEST-dependent degradation appears to occur in the bud after vacuoles arrive in the bud. [2] [3]


Protein Function/Process

Vac17p is involved in vacuole inheritance. Deletion of VAC17 gene blocks vacuole inheritance. Vac17p functions as the vacuole-specific Myp2p receptor. It forms a complex with Myo2p and Vac8p, a vacuolar membrane protein. Vac17p connects Myo2p to Vac8p and thus the vacuole membrane. [2] [3]


Protein Localization

Vac17p is localized on the vacuole membrane in a Vac8p-dependent manner. [2] [3]


References

See Help:References on how to add references

  1. Ishikawa K, et al. (2003) Identification of an organelle-specific myosin V receptor. J Cell Biol 160(6):887-97 SGD PMID 12642614
  2. 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 Tang F, et al. (2003) Regulated degradation of a class V myosin receptor directs movement of the yeast vacuole. Nature 422(6927):87-92 SGD PMID 12594460 Cite error: Invalid <ref> tag; name "S000072665" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000072665" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000072665" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000072665" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000072665" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000072665" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000072665" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000072665" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000072665" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000072665" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000072665" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000072665" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000072665" defined multiple times with different content Cite error: Invalid <ref> tag; name "S000072665" defined multiple times with different content
  3. 3.00 3.01 3.02 3.03 3.04 3.05 3.06 3.07 3.08 3.09 3.10 3.11 3.12 3.13 submitted by Fusheng Tang on 2003-03-17

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