YBR220C

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Systematic name YBR220C
Gene name
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr II:664677..662995
Primary SGDID S000000424


Description of YBR220C: Putative protein of unknown function; YBR220C is not an essential gene[1]




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Community Commentary

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This gene is part of the UW-Stout Orphan Gene Project. Learn more here.

Growth Curve

YBR220C YPD.png

In a BY4735 background, knocking out YBR220C seems to have a moderate effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 414 minutes, while the YBR220C knock-out strain's doubling time was 457 minutes. (These doubling times are the means of three experiments.)

Caffeine and Yeast Cells

megan25.png

This graph shows the growth curve with and without caffeine. It has the knockout strain and wild type yeast cells. The caffeine enhanced the growth in both wild type and knockout strain. The average doubling time between the three experiments for the knockout strand was 825.71 minutes. The caffeine also had a positive effect on the growth curve for the wild type with the average doubling time being 1245.02 minutes. The results were concluded from the wild type and knockout strand was with this amount of caffeine it leads to an increase in growth.

The protocol can be found at

Caffeine

yBR220cUV Exposure.png

This bar graph shows that compared to a BY4735 background, Knocking out yBR220c makes yeast less sensitive to UV light exposure

The Protocol can be found at

UV Light Exposure

Cycloheximide effect on YBR220C

Cycloheximide 7.png

Growth models 1-3 are the yeast strands YBR220C not being stressed. And tests 1-4 are the yeast strands being distressed, 1 is low and is not doing well. 2&3 are a little lower than the growth rate. test 4 is much higher than all.


Effect of 3% DMSO on YBR220C

Effect of 3% DMSO on YBR220C.png

The graph above shows the effect of 3% DMSO on YBR220C. While examining the data from test 3 it is thought that the 3% DMSO test on YBR220C was contaminated; the results are still shown on the graph but the information was not used during growth rate calculations. From the data represented the average doubling rate for the YBR220C knockout strain without DMSO was 652 minutes. When DMSO is added the average doubling time increased to 1019 minutes. This shows that 3% DMSO did add stress to this knockout strain. The average doubling time of WT:BY4735 without DMSO was 514 minutes. The average doubling rate for WT:BY4735 with DMSO was 1529 minutes. If we compare the average doubling rate of WT:BY4735 with DMSO to YBR220C with DMSO we can see that without the YBR220C gene the yeast was able to grow more quickly under DMSO induced stress.

Ethanol Tolerance in YBR220C

YBR220C ethonal.png

This graph shows the growth between YBR220C treated and untreated with ethanol. The rate of growth of the treated yeast is (answer). The rate of growth of the untreated yeast is (answer). This shows that the strands of YBR220C that were treated with ethanol had an increased rate of growth compared to those that weren't.

Prednisone Effects on YBR220C

YBR220C.png



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References

See Help:References on how to add references

  1. Giaever G, et al. (2002) Functional profiling of the Saccharomyces cerevisiae genome. Nature 418(6896):387-91 SGD PMID 12140549

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