Difference between revisions of "YBR033W"

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This graph shows the growth curve with and without caffeine. It has the knockout strain and wild type yeast cells. The caffeine enhanced the growth in both wild type and knockout strain. The average doubling time between the three experiments for the knockout strand was 708.67 minutes. The caffeine also had a positive effect on the growth curve for the wild type with the average doubling time being 1245.02 minutes. The results were concluded from the wild type and knockout strand was with this amount of caffeine it leads to an increase in growth. 
  
 
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==References==
 
==References==
 
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Revision as of 06:43, 12 December 2019

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Systematic name YBR033W
Gene name EDS1
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr II:301944..304703
Primary SGDID S000000237


Description of YBR033W: Putative zinc cluster protein, predicted to be a transcription factor; YBR033W is not an essential gene[1][2]




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Community Commentary

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Growth in YPD

YBR033W YPD.png

In a BY4735 background, knocking out YBR033W seems to have a moderate effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 414 minutes, while the YBR033W knock-out strain's doubling time was 532 minutes. (These doubling times are the means of three experiments.)

Caffeine and Yeast Cells

megan22.png

This graph shows the growth curve with and without caffeine. It has the knockout strain and wild type yeast cells. The caffeine enhanced the growth in both wild type and knockout strain. The average doubling time between the three experiments for the knockout strand was 708.67 minutes. The caffeine also had a positive effect on the growth curve for the wild type with the average doubling time being 1245.02 minutes. The results were concluded from the wild type and knockout strand was with this amount of caffeine it leads to an increase in growth.





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References

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  1. MacPherson S, et al. (2006) A fungal family of transcriptional regulators: the zinc cluster proteins. Microbiol Mol Biol Rev 70(3):583-604 SGD PMID 16959962
  2. Yang Y, et al. (2010) Identifying cooperative transcription factors by combining ChIP-chip data and knockout data. Cell Res 20(11):1276-8 SGD PMID 20975739

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