Difference between revisions of "YBL113C"
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Revision as of 16:30, 5 May 2022
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|Feature type||ORF, Uncharacterized|
Description of YBL113C: Helicase-like protein encoded within the telomeric Y' element
- 1 Community Commentary
- 2 References
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This gene is part of the UW-Stout Orphan Gene Project. Learn more here.
In the results of the 35% dilution of D2O with the Knock-out Yeast Strain YBL113C (A9) we can see that the growth curve stayed in the 0.1-0.3 range throughout the data collected in 24 hours. This tells us it was more sensitive to the dilution of D2O.
As part of the University of Wisconsin Stout Orphan Gene Project this gene was tested by exposing the cells to heat shock.
From the data gathered, there was a medium positive effect to knocking out this gene when it came to the yeast's ability to hold up to heat shock. The modified yeast cells were 252% more resistant to heat shock than the control.
This gene produced 40.50% of the amount of ethanol that the wild type produced.
As part of the University of Wisconsin Stout Orphan Gene Project this gene was tested by exposing the cells to 4mM of caffeine.
- BY4735(wild type yeast) and YBL113C(knockout yeast gene) growth after being subjected to 4mM caffeine solution.
From the growth curve, we can see a clear difference between the rates at which the Wild-Type and YBL113C showed growth. At the same concentration of 4mM caffeine, YBL113 showed greatly reduced growth implying a greater sensitivity to caffeine.
As part of the University of Wisconsin Stout Orphan Gene Project this gene was tested by exposing the cells to hydrogen peroxide.
BY4735 and YBL113C after being exposed to 0.065% dilution of hydrogen peroxide solution.
From the growth curve, we can see that the knockout strain followed a similar sensitivity pattern to the wild type strain, suggesting that the grow has inhibited identically.
See Help:References on how to add references
- Shiratori A, et al. (1999) Systematic identification, classification, and characterization of the open reading frames which encode novel helicase-related proteins in Saccharomyces cerevisiae by gene disruption and Northern analysis. Yeast 15(3):219-53 SGD PMID 10077188
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-According to the graph above, we can see that the KO yeast cell YBL113C follow a smaller sensitivity to the stress than the Wild type strain.
-We can then conclude that the knocked-out gene in YBL113C doesn't have a significant impact on the growth of the yeast cell.