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|Feature type||ORF, Uncharacterized|
Description of YBL086C: Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
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In a BY4735 background, knocking out YBL086C seems to have little or no effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 414 minutes, while the YAL061 knock-out strain's doubling time was 495 minutes. (These doubling times are the means of three experiments.)
Caffeine and Yeast Cells
This graph shows the growth curve with and without caffeine. It has the knockout strain and wild type yeast cells. The caffeine enhanced the growth in both wild type and knockout strain. The average doubling time between the three experiments for the knockout strand was 743.32 minutes. The caffeine also had a positive effect on the growth curve for the wild type with the average doubling time being 1245.02 minutes. The results were concluded from the wild type and knockout strand was with this amount of caffeine it leads to an increase in growth.
The protocol can be found at
This bar graph Shows that compared to a BY4735 background, Knocking out yBL086c seems to make the yeast more sensitive to UV light exposure.
Ethanol Tolerance in YBL086C
This graph shows that there isn't a significant change in growth due to ethanol tolerance. The Knockout strands that were treated with yeast were very similar to the ones that weren't. The doubling rate of the treated YBL086C is (answer). And the doubling rate of the unstressed YBL086C is (answer). This shows that the strands treated with ethanol had an increased growth rate than those that weren't.
See Help:References on how to add references
- Huh WK, et al. (2003) Global analysis of protein localization in budding yeast. Nature 425(6959):686-91 SGD PMID 14562095
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