Difference between revisions of "YBL081W"

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(Cycloheximide on Yeast strand YBL081W)
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This graph shows the growth of YBL081 treated and untreated with Ethanol. The strands of YBL081 that were treated with ethanol had an increase in growth compared to those that weren't. The doubling time of YBL081 that was treated with ethanol is (answer). And the doubling time of the strands that weren't is (answer). This shows that Ethanol leads to an increase in growth in YBL081W.  
 
This graph shows the growth of YBL081 treated and untreated with Ethanol. The strands of YBL081 that were treated with ethanol had an increase in growth compared to those that weren't. The doubling time of YBL081 that was treated with ethanol is (answer). And the doubling time of the strands that weren't is (answer). This shows that Ethanol leads to an increase in growth in YBL081W.  
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===[[UW-Stout/Prednisone_FA19|Prednisone Effects on YBL081W]]===
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[[File:YBL081W.png|750 px]]
  
 
<!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation -->
 
<!-- PLEASE ADD Community Commentary ABOVE THIS MESSAGE. See below for an example of community annotation -->

Revision as of 15:12, 12 December 2019

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Systematic name YBL081W
Gene name
Aliases
Feature type ORF, Uncharacterized
Coordinates Chr II:71866..72972
Primary SGDID S000000177


Description of YBL081W: Non-essential protein of unknown function; null mutation results in a decrease in plasma membrane electron transport[1][2]




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Community Commentary

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This gene is part of the UW-Stout Orphan Gene Project. Learn more here.

Growth in YPD

YBL081W YPD.png

In a BY4735 background, knocking out YBL081W seems to have a moderate effect on growth rate in log-phase. In this assay, the BY4735 strain's doubling time was 414 minutes, while the YAL061 knock-out strain's doubling time was 519 minutes. (These doubling times are the means of three experiments.)

Caffeine and Yeast Cells

megan23.png

This graph shows the growth curve with and without caffeine. It has the knockout strain and wild type yeast cells. The caffeine enhanced the growth in both wild type and knockout strain. The average doubling time between the three experiments for the knockout strand was 741.26 minutes. The caffeine also had a positive effect on the growth curve for the wild type with the average doubling time being 1245.02 minutes. The results were concluded from the wild type and knockout strand was with this amount of caffeine it leads to an increase in growth.

The protocol can be found at

Caffeine

yBL081wUV Exposure.png

This bar graph show that compared to a BY4735 backgroung, Knocking out yBL081w make yeast more sensitive to UV light exposure

Cycloheximide on Yeast strand YBL081W

Cycloheximide 4.png

As we can tell from the figure above that Test 1 did not do well under the stress but tests 2-4 were very happy. The growth Models 1-3 are what the yeasts growth model is not stressed.

Ethanol Tolerance in YBL081W

YBL081W ethonal.png

This graph shows the growth of YBL081 treated and untreated with Ethanol. The strands of YBL081 that were treated with ethanol had an increase in growth compared to those that weren't. The doubling time of YBL081 that was treated with ethanol is (answer). And the doubling time of the strands that weren't is (answer). This shows that Ethanol leads to an increase in growth in YBL081W.

Prednisone Effects on YBL081W

YBL081W.png



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References

See Help:References on how to add references

  1. Giaever G, et al. (2002) Functional profiling of the Saccharomyces cerevisiae genome. Nature 418(6896):387-91 SGD PMID 12140549
  2. Herst PM, et al. (2008) Plasma membrane electron transport in Saccharomyces cerevisiae depends on the presence of mitochondrial respiratory subunits. FEMS Yeast Res 8(6):897-905 SGD PMID 18657191

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