UW Stout/Lithium chloride FA19

Safety Precautions: Safety Goggles Gloves Lab Coat Work under hood

Materials: 96 plate Yeast strands H20 Lithium Chloride 250ml beaker 10, 1.7ml microcentrifuge tube

Protocol:

Make a solution of lithium chloride with 4.24g of LiCl and 9mL of H20, then adjust the volume to 1mL of H20 in a 100ml beaker Filter sterilize with .45 micron syringe filter Make 11 different concentration in 1.7ml microcentrifuge tubes with 20ul of H2O starting at 0.5% concentration increasing to a 5.5% concentration Pipette 5 u from solution 1 into the second well on the 96 plates, and the second solution into the second well, until each solution is in each well. In the first well add 10ul of H20 without any LiCl solution