Difference between revisions of "UW Stout/Lithium chloride FA19"
| Line 16: | Line 16: | ||
Protocol: | Protocol: | ||
| − | 1.Make a solution of lithium chloride with 4.24g of LiCl and 9mL of H20, then adjust the volume to 1mL of H20 in a 100ml beaker | + | 1. Make a solution of lithium chloride with 4.24g of LiCl and 9mL of H20, then adjust the volume to 1mL of H20 in a 100ml beaker |
| − | 2.Filter sterilize with .45 micron syringe filter | + | |
| − | 3.Make 11 different concentration in 1.7ml microcentrifuge tubes with 20ul of H2O starting at 0.5% concentration increasing to a 5.5% concentration | + | 2. Filter sterilize with .45 micron syringe filter |
| + | |||
| + | 3. Make 11 different concentration in 1.7ml microcentrifuge tubes with 20ul of H2O starting at 0.5% concentration increasing to a 5.5% concentration | ||
| + | |||
4.Pipette 5 u from solution 1 into the second well on the 96 plates, and the second solution into the second well, until each solution is in each well. | 4.Pipette 5 u from solution 1 into the second well on the 96 plates, and the second solution into the second well, until each solution is in each well. | ||
| − | 5.In the first well add 10ul of H20 without any LiCl solution | + | |
| + | 5. In the first well add 10ul of H20 without any LiCl solution | ||
[[File:liclconcentraion!.png]] | [[File:liclconcentraion!.png]] | ||
Revision as of 09:01, 18 December 2019
Safety Precautions: Safety Goggles Gloves Lab Coat Work under hood
Materials: 96 plate Yeast strands H20 Lithium Chloride 250ml beaker 10, 1.7ml microcentrifuge tube
Protocol:
1. Make a solution of lithium chloride with 4.24g of LiCl and 9mL of H20, then adjust the volume to 1mL of H20 in a 100ml beaker
2. Filter sterilize with .45 micron syringe filter
3. Make 11 different concentration in 1.7ml microcentrifuge tubes with 20ul of H2O starting at 0.5% concentration increasing to a 5.5% concentration
4.Pipette 5 u from solution 1 into the second well on the 96 plates, and the second solution into the second well, until each solution is in each well.
5. In the first well add 10ul of H20 without any LiCl solution
