UW Stout/Formamide FA19
Haley and Thomas
Formamide Sensitivity Torture Experiment
Written by Haley Monette and Thomas Lo
Reasoning: To observe the growth of the yeast cells by adding different concentrations of formamide.
Background: Since previous experiments succeeded with the concentration of the formamide being 3%, we decided to test a range surrounding that mid-point.
We will test .5 increments above and below 3%, ranging from .5%-5.5%, measuring 12 concentrations.
Neg Control- No formamide meaning 50 ul yeast and 50 ul water
- 600ml of concentrated yeast cell solution (OD 600 0.2)
- Assay plate (has 96 wells)
- “Neat” (full conc.) formamide 33 ul
- PCR Tube
- 567ul of H2O
- Fume Hood
- Incubator set to 30 degrees Celsius
Excessive inhalation of vapor may cause symptoms that parallel ingestion, ranging from headache to unconsciousness, depending upon the duration and level of the exposure. May cause headache, dizziness, nausea, vomiting, abdominal pain, and unconsciousness.
- Make sure the H2O is sterile
- Make sure everything you use in the experiment is sterile, including yourself (spray everything down with 70% ethanol)
- Formamide is best accessible when taking 40 ul and putting it in a separate tube, apart from the bottle
- Make sure to use a different tip each time you grab water, yeast, or formamide
- Return bottle of formamide to frig when done
- Grab the bottle of neat formamide and pipet out 36 ul into a PCR Tube.
- Obtain 561 ul of H2O in a beaker
- In the fume hood, dilute out each yeast solution with desired formamide concentration. *Refer to chart below (Desired formamide concentration mixtures)