UW Stout/Formamide FA19
Haley and Thomas
Formamide Sensitivity Torture Experiment
Written by Haley Monette and Thomas Lo
Reasoning: To observe the growth of the yeast cells by adding different concentrations of formamide.
Background: Since previous experiments succeeded with the concentration of the formamide being 3%, we decided to test a range surrounding that mid-point.
We will test .5 increments above and below 3%, ranging from .5%-5.5%, measuring 12 concentrations.
Neg Control- No formamide meaning 50 ul yeast and 50 ul water
- 600ml of concentrated yeast cell solution (OD 600 0.2)
- Assay plate (has 96 wells)
- “Neat” (full conc.) formamide 33 ul
- PCR Tube
- 567ul of H2O
- Fume Hood
- Incubator set to 30 degrees Celsius