Difference between revisions of "UW Stout/Formamide FA19"
| Line 17: | Line 17: | ||
'''Materials:''' | '''Materials:''' | ||
| − | *600ml of concentrated yeast cell solution (OD 600 0.2 | + | *600ml of concentrated yeast cell solution (OD 600 0.2) |
| + | *Assay plate (has 96 wells) | ||
| + | *“Neat” (full conc.) formamide 33 ul | ||
Revision as of 11:06, 10 December 2019
Haley and Thomas
Formamide Sensitivity Torture Experiment
Written by Haley Monette and Thomas Lo
Reasoning: To observe the growth of the yeast cells by adding different concentrations of formamide.
Background: Since previous experiments succeeded with the concentration of the formamide being 3%, we decided to test a range surrounding that mid-point.
We will test .5 increments above and below 3%, ranging from .5%-5.5%, measuring 12 concentrations.
Neg Control- No formamide meaning 50 ul yeast and 50 ul water
Materials:
- 600ml of concentrated yeast cell solution (OD 600 0.2)
- Assay plate (has 96 wells)
- “Neat” (full conc.) formamide 33 ul
