Difference between revisions of "UW Stout/Formamide FA19"
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Revision as of 11:11, 10 December 2019
Haley and Thomas
Formamide Sensitivity Torture Experiment
Written by Haley Monette and Thomas Lo
Reasoning: To observe the growth of the yeast cells by adding different concentrations of formamide.
Background: Since previous experiments succeeded with the concentration of the formamide being 3%, we decided to test a range surrounding that mid-point.
We will test .5 increments above and below 3%, ranging from .5%-5.5%, measuring 12 concentrations.
Neg Control- No formamide meaning 50 ul yeast and 50 ul water
Materials:
- 600ml of concentrated yeast cell solution (OD 600 0.2)
- Assay plate (has 96 wells)
- “Neat” (full conc.) formamide 33 ul
- PCR Tube
- 567ul of H2O
- Fume Hood
- Micropipette
- Incubator set to 30 degrees Celsius
CAUTION:
Excessive inhalation of vapor may cause symptoms that parallel ingestion, ranging from headache to unconsciousness, depending upon the duration and level of the exposure. May cause headache, dizziness, nausea, vomiting, abdominal pain, and unconsciousness.
