Difference between revisions of "UW Stout/Formamide FA19"

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*Assay plate (has 96 wells)
*Assay plate (has 96 wells)
*“Neat” (full conc.) formamide 33 ul
*“Neat” (full conc.) formamide 33 ul
*PCR Tube
*567ul of H2O
*Fume Hood
*Incubator set to 30 degrees Celsius

Revision as of 11:09, 10 December 2019

Haley and Thomas

Formamide Sensitivity Torture Experiment

                             Written by Haley Monette and Thomas Lo

Reasoning: To observe the growth of the yeast cells by adding different concentrations of formamide.

Background: Since previous experiments succeeded with the concentration of the formamide being 3%, we decided to test a range surrounding that mid-point.

We will test .5 increments above and below 3%, ranging from .5%-5.5%, measuring 12 concentrations.

Neg Control- No formamide meaning 50 ul yeast and 50 ul water


  • 600ml of concentrated yeast cell solution (OD 600 0.2)
  • Assay plate (has 96 wells)
  • “Neat” (full conc.) formamide 33 ul
  • PCR Tube
  • 567ul of H2O
  • Fume Hood
  • Micropipette
  • Incubator set to 30 degrees Celsius