UW Stout/Ethanol FA19
Yeast torture 1 protocol: Materials:
- neat ethanol
- yeast
- sterile water
- 200 ul
- 96 well plate
Procedure:
- (add 50ul mixture to 50ul yeast in 96 well plate.)x2. Used 12 wells in total
- fill 6 200ul tubes, each with water and ethanol concentration x2. Resulting in 6 200ul tubes with 100ul of mixture in each.
| 1 | 2 | 3 | 4 | 5 | 6 | |
|---|---|---|---|---|---|---|
| ul ethanol | 0 | 3.75 | 7 | 10.25 | 13.5 | 16.75 |
| ul water | 50 | 46.25 | 43 | 39.75 | 37.5 | 33.25 |
| ul yeast | 50 | 50 | 50 | 50 | 50 | 50 |
