Difference between revisions of "UW Stout/Ethanol FA19"
Line 47: | Line 47: | ||
===Protocol:=== | ===Protocol:=== | ||
Materials: | Materials: | ||
− | + | #Yeast Knockout Strains: | |
##YAL061W | ##YAL061W | ||
##YBL086C | ##YBL086C | ||
Line 59: | Line 59: | ||
##YKR005C | ##YKR005C | ||
##WT:BY4735 | ##WT:BY4735 | ||
− | + | #neat ethanol | |
− | + | #sterile water | |
− | + | #micropipeter(s) | |
− | + | #96 well plate | |
Procedure: | Procedure: | ||
#add 50 ul of Knockout Strain to individual well (96 well plate). | #add 50 ul of Knockout Strain to individual well (96 well plate). |
Revision as of 10:52, 10 December 2019
Yeast torture 1
Protocol:
Materials:
- neat ethanol
- yeast
- sterile water
- 200 ul
- 96 well plate
Procedure:
- (add 50ul mixture to 50ul yeast in 96 well plate.)x2. Used 12 wells in total
- fill 6 200ul tubes, each with water and ethanol concentration x2. Resulting in 6 200ul tubes with 100ul of mixture in each.
1 | 2 | 3 | 4 | 5 | 6 | |
---|---|---|---|---|---|---|
ul ethanol | 0 | 3.75 | 7 | 10.25 | 13.5 | 16.75 |
ul water | 50 | 46.25 | 43 | 39.75 | 37.5 | 33.25 |
ul yeast | 50 | 50 | 50 | 50 | 50 | 50 |
Knockout Strand Yeast Torture
Protocol:
Materials:
- Yeast Knockout Strains:
- YAL061W
- YBL086C
- YBL9081W
- YBR033W
- YBR138C
- YBR220C
- YHR048W
- YGL140C
- YJR061W
- YKR005C
- WT:BY4735
- neat ethanol
- sterile water
- micropipeter(s)
- 96 well plate
Procedure:
- add 50 ul of Knockout Strain to individual well (96 well plate).
- add 50 ul of water+ethanol mixture to individual well (Follow table below).
Well | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 |
---|---|---|---|---|---|---|---|---|---|---|---|---|
ul yeast | 50 | 50 | 50 | 50 | 50 | 50 | 50 | 50 | 50 | 50 | 50 | 50 |
ul ethanol | 7 | 7 | 7 | 7 | 7 | 7 | 7 | 7 | 7 | 7 | 7 | 7 |
ul water | 43 | 43 | 43 | 43 | 43 | 43 | 43 | 43 | 43 | 43 | 43 | 43 |