Difference between revisions of "UW Stout/Ethanol FA19"

From SGD-Wiki
Jump to: navigation, search
(Created page with "Yeast torture 1 protocol: Materials: # neat ethanol # yeast # sterile water # 200 ul # 96 well plate Procedure: # (add 50ul mixture to 50ul yeast in 96 well plate.)x2. Used...")
 
Line 1: Line 1:
−
Yeast torture 1 protocol:
+
==Yeast torture 1==
 +
===Protocol:===
 
Materials:  
 
Materials:  
 
# neat ethanol
 
# neat ethanol
Line 42: Line 43:
 
| 50
 
| 50
 
| 50
 
| 50
 +
|}
 +
==Knockout Strand Yeast Torture===
 +
===Protocol:===
 +
Materials:
 +
*Yeast Knockout Strains:
 +
##YAL061W
 +
##YBL086C
 +
##YBL9081W
 +
##YBR033W
 +
##YBR138C
 +
##YBR220C
 +
##YHR048W
 +
##YGL140C
 +
##YJR061W
 +
##YKR005C
 +
##WT:BY4735
 +
{| class="wikitable"
 +
! yeast
 +
! 1
 +
! 2
 +
! 3
 +
! 4
 +
! 5
 +
! 6
 +
! 7
 +
! 8
 +
! 9
 +
! 10
 +
! 11
 +
! 12
 +
|-
 +
| ul yeast
 +
| 50
 +
| 50
 +
| 50
 +
| 50
 +
| 50
 +
| 50
 +
| 50
 +
| 50
 +
| 50
 +
| 50
 +
| 50
 +
| 50
 +
|-
 +
| ul ethanol
 +
| 7
 +
| 7
 +
| 7
 +
| 7
 +
| 7
 +
| 7
 +
| 7
 +
| 7
 +
| 7
 +
| 7
 +
| 7
 +
| 7
 +
|-
 +
| ul water
 +
| 43
 +
| 43
 +
| 43
 +
| 43
 +
| 43
 +
| 43
 +
| 43
 +
| 43
 +
| 43
 +
| 43
 +
| 43
 +
| 43
 
|}
 
|}

Revision as of 10:42, 10 December 2019

Yeast torture 1

Protocol:

Materials:

  1. neat ethanol
  2. yeast
  3. sterile water
  4. 200 ul
  5. 96 well plate

Procedure:

  1. (add 50ul mixture to 50ul yeast in 96 well plate.)x2. Used 12 wells in total
  2. fill 6 200ul tubes, each with water and ethanol concentration x2. Resulting in 6 200ul tubes with 100ul of mixture in each.
1 2 3 4 5 6
ul ethanol 0 3.75 7 10.25 13.5 16.75
ul water 50 46.25 43 39.75 37.5 33.25
ul yeast 50 50 50 50 50 50

Knockout Strand Yeast Torture=

Protocol:

Materials:

  • Yeast Knockout Strains:
    1. YAL061W
    2. YBL086C
    3. YBL9081W
    4. YBR033W
    5. YBR138C
    6. YBR220C
    7. YHR048W
    8. YGL140C
    9. YJR061W
    10. YKR005C
    11. WT:BY4735
yeast 1 2 3 4 5 6 7 8 9 10 11 12
ul yeast 50 50 50 50 50 50 50 50 50 50 50 50
ul ethanol 7 7 7 7 7 7 7 7 7 7 7 7
ul water 43 43 43 43 43 43 43 43 43 43 43 43
Retrieved from "https://wiki.yeastgenome.org/index.php?title=UW_Stout/Ethanol_FA19&oldid=401552"