This page is dedicated to the test protocol used to view stress on yeast cells in a lowered pH environment. We startded by mixing up 1 molar HCL into distilled water ussing a pH probe to determin when nwe reached the desired level. We had pH from 7 down to 4 goin in roughly 1 pH step per solution. We the ussed these in a calibration test where we tested a unmodified wild typ BY4735. This gave us a data sheet showing the growth times and effects of the varied pH levels.