Difference between revisions of "UW-Stout/Saline SP23"

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'''Saltwater Torture'''
 
'''Saltwater Torture'''
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'''Pilot Experiment'''
  
 
'''Introduction''':  
 
'''Introduction''':  
This experiment is testing how much salt will stress out the yeast without killing them. When we did our pilot experiment, we found out that 2 M saltwater has a differing effect on the growth of yeast cells with different genes edited in.  
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This experiment is testing how much salt will stress out the yeast without killing them. Our goal of this experiment is to see how much salt is needed to allow the yeast to grow while altering their growth curve without killing them. In this pilot experiment we tested wild yeast strains against different molarities of salt water. Looking at the growth curve data will help us determine which molarity of salt water stressed out the yeast without killing them.  
 
 
'''Materials'''
 
*H2O
 
*NaCl (table salt)
 
*2 M saltwater
 
*micropipettes
 
*Yeast cells (wild, 1, 2, & 3)
 
*Microwell plate
 
  
'''Procedure''':
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'''Materials''':  
#Obtain a sample of 2 M saltwater.
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*H2O*
#Obtain the yeast cells
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*NaCl (table salt)*
#Pick a row in the microwell plate and micropipette 50 ul of 2 M saltwater into the first four wells of the row.
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*12 2 ml centrifuge tubes* 
#Micropipette 50 ul of each of the yeast cells into the wells with the saltwater.
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*Yeast cells*
#Record where you put each sample.
 
#Put into plate reader for 18-24 hours.
 
#Obtain results from plate reader and calculate the doubling rate.
 

Revision as of 16:44, 6 May 2023

Saltwater Torture

Pilot Experiment

Introduction: This experiment is testing how much salt will stress out the yeast without killing them. Our goal of this experiment is to see how much salt is needed to allow the yeast to grow while altering their growth curve without killing them. In this pilot experiment we tested wild yeast strains against different molarities of salt water. Looking at the growth curve data will help us determine which molarity of salt water stressed out the yeast without killing them.

Materials:

  • H2O*
  • NaCl (table salt)*
  • 12 2 ml centrifuge tubes*
  • Yeast cells*