Difference between revisions of "UW-Stout/Saline SP23"
| Line 3: | Line 3: | ||
'''Introduction''': | '''Introduction''': | ||
| − | This experiment is testing how 2 M saltwater has a differing effect on the growth of yeast cells with different genes edited in. | + | This experiment is testing how much salt will stress out the yeast without killing them. When we did our pilot experiment, we found out that 2 M saltwater has a differing effect on the growth of yeast cells with different genes edited in. |
'''Materials''' | '''Materials''' | ||
Revision as of 16:36, 6 May 2023
Saltwater Torture
Introduction: This experiment is testing how much salt will stress out the yeast without killing them. When we did our pilot experiment, we found out that 2 M saltwater has a differing effect on the growth of yeast cells with different genes edited in.
Materials
- H2O
- NaCl (table salt)
- 2 M saltwater
- micropipettes
- Yeast cells (wild, 1, 2, & 3)
- Microwell plate
Procedure:
- Obtain a sample of 2 M saltwater.
- Obtain the yeast cells
- Pick a row in the microwell plate and micropipette 50 ul of 2 M saltwater into the first four wells of the row.
- Micropipette 50 ul of each of the yeast cells into the wells with the saltwater.
- Record where you put each sample.
- Put into plate reader for 18-24 hours.
- Obtain results from plate reader and calculate the doubling rate.
