UW-Stout/Methanol

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Yeast Growth In Methanol

"Protocol"

Materials:

  • yeast
  • methanol

"Equipment"

  • assay plate
  • 30 degree c incubator

Strains of Yeast

  • By4735 (wild type)
  • YEL035c
  • YCL002c
  • YFL064c
  • YGL235w
  • YCR100c
  • YJL043w
  • YPL068c
  • YMR090w

We added 80 microliters of each different strand of yeast into the wells and then added 20 microliters of methanol to give a total of 100 microliters in each well with a 20% concentration.

Put the assay plate in a 30 degrees Celsius incubator and shook it at 120 revolutions/ min for

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