Difference between revisions of "UW-Stout/Methanol"
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'''Strains of Yeast''' | '''Strains of Yeast''' | ||
| + | ''experiment 3'' | ||
* By4735 (wild type) | * By4735 (wild type) | ||
* YEL035c | * YEL035c | ||
| Line 25: | Line 26: | ||
Put the assay plate in a 30 degrees Celsius incubator and shook it at 120 revolutions/ min for | Put the assay plate in a 30 degrees Celsius incubator and shook it at 120 revolutions/ min for | ||
| + | |||
| + | ==Results From Experiment 1== | ||
| + | |||
| + | * 90 microliters of yeast + 10 microliters of methanol: 86 min | ||
| + | * 90 microliters of yeast + 10 microliters of water: 72 min | ||
| + | * 90 microliters of yeast + 5 microliters of methanol + 5 microliters of water: 76 min | ||
| + | * 90 microliters of yeast + 2 microliters of methanol + 8 microliters of water: 73 min | ||
| + | * 90 microliters of yeast + 1 microliter of methanol + 1 microliter of water: 78 min | ||
| + | * mix 9.5 microliters water and .5 microliters of methanol in a separate tube, flick to mix and add to 90 microliters of yeast: 67 min | ||
| + | * mix 9.75 microliters of water and .25 microliters of methanol in a separate tube, flick to mix and add to 90 microliters of yeast: 128 min | ||
| + | * mix 9.88 microliters of water and .125 microliters of methanol in a separate tube, flick to mix and add to 90 microliters of yeast: 87 min | ||
| + | * mix 9.95 microliters of water and .05 microliters of methanol in a separate tube, flick to mix and add to 90 microliters of yeast: 86 min | ||
| + | * mix 9.98 microliters of water and .02 microliters of methanol in a separate tube, flick to mix and add to 90 microliters of yeast: 84 min | ||
| + | * mix 9.99 microliters of water and .01 microliters of methanol in a separate tube, flick to mix and add to 90 microliters of yeast: 92 min | ||
| + | * mix 9.999 microliters of water and .001 microliters of methanol in a separate tube, flick to mix and add to 90 microliters of yeast: 85 min | ||
Revision as of 07:05, 30 April 2019
Yeast Growth In Methanol
Protocol
Materials:
- yeast
- methanol
Equipment
- assay plate
- 30 degree c incubator
Strains of Yeast experiment 3
- By4735 (wild type)
- YEL035c
- YCL002c
- YFL064c
- YGL235w
- YCR100c
- YJL043w
- YPL068c
- YMR090w
We added 80 microliters of each different strand of yeast into the wells and then added 20 microliters of methanol to give a total of 100 microliters in each well with a 20% concentration.
Put the assay plate in a 30 degrees Celsius incubator and shook it at 120 revolutions/ min for
Results From Experiment 1
- 90 microliters of yeast + 10 microliters of methanol: 86 min
- 90 microliters of yeast + 10 microliters of water: 72 min
- 90 microliters of yeast + 5 microliters of methanol + 5 microliters of water: 76 min
- 90 microliters of yeast + 2 microliters of methanol + 8 microliters of water: 73 min
- 90 microliters of yeast + 1 microliter of methanol + 1 microliter of water: 78 min
- mix 9.5 microliters water and .5 microliters of methanol in a separate tube, flick to mix and add to 90 microliters of yeast: 67 min
- mix 9.75 microliters of water and .25 microliters of methanol in a separate tube, flick to mix and add to 90 microliters of yeast: 128 min
- mix 9.88 microliters of water and .125 microliters of methanol in a separate tube, flick to mix and add to 90 microliters of yeast: 87 min
- mix 9.95 microliters of water and .05 microliters of methanol in a separate tube, flick to mix and add to 90 microliters of yeast: 86 min
- mix 9.98 microliters of water and .02 microliters of methanol in a separate tube, flick to mix and add to 90 microliters of yeast: 84 min
- mix 9.99 microliters of water and .01 microliters of methanol in a separate tube, flick to mix and add to 90 microliters of yeast: 92 min
- mix 9.999 microliters of water and .001 microliters of methanol in a separate tube, flick to mix and add to 90 microliters of yeast: 85 min
