Difference between revisions of "UW-Stout/Methanol"
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==Yeast Growth In Methanol== | ==Yeast Growth In Methanol== | ||
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'''Materials:''' | '''Materials:''' | ||
Revision as of 06:46, 30 April 2019
Yeast Growth In Methanol
Protocol
Materials:
- yeast
- methanol
Equipment
- assay plate
- 30 degree c incubator
Strains of Yeast
- By4735 (wild type)
- YEL035c
- YCL002c
- YFL064c
- YGL235w
- YCR100c
- YJL043w
- YPL068c
- YMR090w
We added 80 microliters of each different strand of yeast into the wells and then added 20 microliters of methanol to give a total of 100 microliters in each well with a 20% concentration.
Put the assay plate in a 30 degrees Celsius incubator and shook it at 120 revolutions/ min for
