Difference between revisions of "UW-Stout/Methanol"
(Created page with "==Yeast Growth In Methanol== "Protocol" Materials: * yeast * methanol * assay plate '''Strains of Yeast''' * By4735 * YEL035c * YCL002c * YFL064c * YGL235w * YCR100c * YJL0...") |
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* yeast | * yeast | ||
* methanol | * methanol | ||
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| + | "Equipment" | ||
* assay plate | * assay plate | ||
| + | * 30 degree c incubator | ||
'''Strains of Yeast''' | '''Strains of Yeast''' | ||
| − | * By4735 | + | * By4735 (wild type) |
* YEL035c | * YEL035c | ||
* YCL002c | * YCL002c | ||
Revision as of 06:45, 30 April 2019
Yeast Growth In Methanol
"Protocol"
Materials:
- yeast
- methanol
"Equipment"
- assay plate
- 30 degree c incubator
Strains of Yeast
- By4735 (wild type)
- YEL035c
- YCL002c
- YFL064c
- YGL235w
- YCR100c
- YJL043w
- YPL068c
- YMR090w
We added 80 microliters of each different strand of yeast into the wells and then added 20 microliters of methanol to give a total of 100 microliters in each well with a 20% concentration.
Put the assay plate in a 30 degrees Celsius incubator and shook it at 120 revolutions/ min for
